Skip to main content

Advertisement

SpringerLink
Log in

Acetylcholine regulates glucagon secretion from human glucagonoma cells

  • Rapid Communication
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

Human glucagonoma cells were isolated and maintained in vitro. Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Atropine abolished the stimulatory effect of Cch on glucagon, VIP, and PP release. An immunohistological study of the tumor tissues revealed that the cells contained glucagon, VIP, and PP. These findings demonstrate, for the first time, the in vitro release of glucagon from glucagonoma cells by Cch stimulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Tanaka T, Nakamura K, Watanabe T, et al. Glucagonoma syndrome (in Japanese). Pathol Clin Med 1984;2:518–524.

    Google Scholar 

  2. Yasunami Y, Funakoshi A, Ono J, et al. In vitro study of cultured human insulinoma cells: Evidence of abnormal sensitivity to glucose. J Clin Endocrinol Metab 1987;65:110–115.

    CAS  PubMed  Google Scholar 

  3. Yasunami Y, Funakoshi A, Ryu S, et al. In vitro release of vasoactive intestinal polypeptide and pancreatic polypeptide from human VIPoma cells and its inhibition by somatostatin analogue SMS 201-995. Surgery 1994;115:713–717.

    CAS  PubMed  Google Scholar 

  4. Nakano I, Funakoshi A, Shinozaki H. et al. Plasma cholecystokinin and pancreatic polypeptide responses after ingestion of a liquid test meal rich in medium-chain fatty acids in patients with chronic pancreatitis. Am J Clin Nutr 1989;49:247–251.

    CAS  PubMed  Google Scholar 

  5. Ishii H, Miyazaki K, Funakoshi A, et al. A case of multiple endocrine neoplasia (MEN) type 1: The immunohistochemical and ultrastructural studies of its tumors and the analysis of hormones in tumor extracts. Endocrinol Jpn 1989;36:37–45.

    CAS  PubMed  Google Scholar 

  6. Schwartz TW. Pancreatic polypeptide: A hormone under vagal control. Gastroenterology 1983;85:1411–1425.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Gastroenterology, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, 815, Fukuoka, Japan

    Akihiro Funakoshi & Hirotsugu Shinozaki

  2. First Department of Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-01, Fukuoka, Japan

    Yohichi Yasunami

  3. First Department of Surgery, Kyushu University Faculty of Medicine, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Shinichiroh Ryu

  4. First Department of Pathology, Kurume University School of Medicine, 67 Asahimachi, 830, Kurume, Japan

    Atsuo Jimi

Authors
  1. Akihiro Funakoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yohichi Yasunami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shinichiroh Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hirotsugu Shinozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Atsuo Jimi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Funakoshi, A., Yasunami, Y., Ryu, S. et al. Acetylcholine regulates glucagon secretion from human glucagonoma cells. J Gastroenterol 29, 797–799 (1994). https://doi.org/10.1007/BF02349291

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02349291

Key words

Search

Navigation