Skip to main content

Advertisement

SpringerLink
Log in

Anti-Idiotypic antibody production in hepatitis B vaccine recipients

  • Liver, Pancreas, and Biliary Tract
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

Antibodies against hepatitis B surface antigen and against anti-hepatitis B surface antigen idiotype were assayed after immunization with hepatitis B vaccine both in sera, by enzyme-linked immunosorbent assay (ELISA), and in peripheral blood mononuclear cells, by enzyme-linked immunospot (ELISPOT) assay. After vaccination of 19 subjects, antibody to the idiotype of anti-hepatitis B surface antigen was detected in none of the sera tested with ELISA, but antiidiotypic antibody-secreting cells were detected by ELISPOT assay in 4 (36.4%) of the 11 vaccine recipients who were positive for anti-hepatitis B surface antigen with ELISPOT assay. On the other hand, these cells were detected in none of those who remained seronegative for anti-hepatitis B surface antigen, or in the 7 normal subjects or the 2 chronic hepatitis type C patients. These results suggest that anti-idiotypic antibody production is more sensitively detected by ELISPOT assay than by ELISA, and anti-idiotypic antibodies to anti-hepatitis B surface antigen may be present in those with anti-hepatitis B surface antigen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Maupas PH, Goudeau A, Cousaget P, et al. Immunization against hepatitis B in man: A pilot study of 2 years' duration. In: Vyas GN (ed) Viral hepatitis. Philadelphia: Franklin Institute Press, 1978;539–556.

    Google Scholar 

  2. Szmuness W, Stevens CE, Harley EJ, et al. The Vaccine Trial Study Group. Hepatitis B vaccine in medical staff of hemodialysis units. Efficacy and subtype cross-protection. New Engl J Med 1982;307:1482–1486.

    Google Scholar 

  3. McLeer W, Buynuk EB, Maigetter RZ, et al. Human hepatitis B vaccine from recombinant yeast. Nature 1984;307:178–180.

    Google Scholar 

  4. Petre J, Van Wijmendaele F, DeNays B, et al. Development of a hepatitis B vaccine from transformed yeast cells. Postgrad Med J 1987;63[Suppl 2]:73–81.

    CAS  PubMed  Google Scholar 

  5. Jilg W, Lorbeer B, Schmidt F, et al. Clinical evaluation of a recombinant hepatitis B vaccine. Lancet 1984;II:1174–1175.

    Google Scholar 

  6. Jilg W, Deinhardt F. Results of immunization with a recombinant yeast-derived hepatitis B vaccine. J Infect 1986;13[Suppl A]:47–51.

    PubMed  Google Scholar 

  7. Cupps TR, Gerin JL, Purcell RH, et al. In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements. J Clin Invest 1984;74:1204–1213.

    CAS  PubMed  Google Scholar 

  8. Milich DR. Genetic and molecular basis for T-and B-cell recognition of hepatitis B viral antigens. Immunol Rev 1987;99:71–103.

    CAS  PubMed  Google Scholar 

  9. Filion LG, Saginur R, Szczerback N. Hunoral and cellular immune response by normal individuals to hepatitis B surface antigen vaccination. Clin Exp Immunol 1988;71:405–409.

    CAS  PubMed  Google Scholar 

  10. Cupps TR, Haas-Smith SA, Gerin JL, et al. Anti-idiotypic modulation of the in vitro immune response to hepatitis B surface antigen (HBsAg) following remote infection by hepatitis B virus in man. Cell Immunol 1989;122:244–250.

    Article  CAS  PubMed  Google Scholar 

  11. Kobayashi K, Suzuki H, Ueno Y, et al. Anti-idiotypic antibodies directed against anti-HBs among patients with chronic hepatitis B. Tohoku J Exp Med 1990;161:261–271.

    CAS  PubMed  Google Scholar 

  12. Anh-Tuan N, Novack E. Detection and quantitation of hepatitis B surface antigen immune complexes (HBsAg-ICs) by an antigen-specific method. I. Detection and quantitation of in vitro prepared HBsAg-ICs. J Immunol Methods 1980;33:193–300.

    Article  Google Scholar 

  13. Czerkinsky CC, Nilsson LA, Nygren H, et al. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods 1983;65:109–121.

    Article  CAS  PubMed  Google Scholar 

  14. Czerkinsky C, Moldoveanu Z, Mestecky J, et al. A novel twocolour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells. J Immunol Methods 1988;115: 31–37.

    Article  CAS  PubMed  Google Scholar 

  15. Rifai A, Wu CG. Clearance kinetics, tissue localization and fate of IgA-antiidiotype complexes. Immunology 1990;69:610–615.

    CAS  PubMed  Google Scholar 

  16. Tankersley DL, Preston MS, Finlayson JS. Immunoglobulin G dimer: An idiotype-anti-idiotype complex. Mol Immunol 1988; 25:41–48.

    Article  CAS  PubMed  Google Scholar 

  17. Cosenza H. Detection of anti-idiotype reactive cells in response to phosphorylcholine. Eur J Immunol 1976;6:114–116.

    CAS  PubMed  Google Scholar 

  18. Koopman WJ, Schrohenloher RE, Barton JC, Greenleaf BC. Suppression of in vitro monoclonal human rheumatoid factor synthesis by anti-idiotypic antibody. Target cells and molecular requirements. J Clin Invest 1983;72:1410–1419.

    CAS  PubMed  Google Scholar 

  19. Zanetti M, Bigazzi PE. Anti-idiotypic immunity and autoimmunity. 1. In vitro and in vivo effects of anti-idioytpic antibodies to spontaneously occurring autoantibodies to rat thyroglobulin. Eur J Immunol 1981;11:187–195.

    CAS  PubMed  Google Scholar 

  20. Pride MW, Thakur A, Thanavala Y. Mimlcry of the a determinant of hepatitis B surface antigen by an antiidiotypic antibody. I. Evaluation in hepatitis B surface antigen responder and nonresponder strains. J Exp Med 1993;177:127–134.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Third Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, 980, Sendai, Japan

    Koju Kobayashi, Yoshiyuki Ueno, Hiroshi Suzuki, Masahito Miura, Ryoichi Nagatomi, Motoyasu Ishii & Takayoshi Toyota

Authors
  1. Koju Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshiyuki Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroshi Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masahito Miura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ryoichi Nagatomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Motoyasu Ishii
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Takayoshi Toyota
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Part of this work was published in abstract form (Gastroenterology 98: A457, 1990) and presented at the annual meeting of the American Gastroenterological Association (1990).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kobayashi, K., Ueno, Y., Suzuki, H. et al. Anti-Idiotypic antibody production in hepatitis B vaccine recipients. J Gastroenterol 29, 740–744 (1994). https://doi.org/10.1007/BF02349280

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02349280

Key words

Search

Navigation