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Radioiodination of recombinant human growth hormone and its use in radioimmunoassay

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Abstract

Radioimmunoassay (RIA) of human growth hormone (hGH) using125I-labeled tracer prepared from DNA recombinant hGH (r-hGH) and characterization of the tracer in the assay system are described. The radioiodination of r-hGH resulted in high yield of immunoreactive tracer. The immunoreactive fraction could be purified by gel-filtration on sephadex G-75. The quality of radioiodinated tracer of r-hGH has been found to be same as that of the tracer obtained from pituitary hGH (p-hGH) with respect to immunoreactivity, assay sensitivity and RIA standard curve parameters.

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Authors and Affiliations

  1. Unit of Department of Atomic Energy, Board of Radiation and Isotope Technology (BRIT), V. N. Purav Marg, Deonar, 400 094, Calcutta, India

    T. K. Karir, S. M. Mirapurkar, N. Jyotsana & N. Sivaprasad

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  1. T. K. Karir
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  2. S. M. Mirapurkar
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  3. N. Jyotsana
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  4. N. Sivaprasad
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Karir, T.K., Mirapurkar, S.M., Jyotsana, N. et al. Radioiodination of recombinant human growth hormone and its use in radioimmunoassay. J Radioanal Nucl Chem 240, 343–347 (1999). https://doi.org/10.1007/BF02349176

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  • DOI: https://doi.org/10.1007/BF02349176

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