Skip to main content
Log in

Apoptosis in the reduced enamel epithelium just after tooth emergence in rats

In situ labeling of nuclear DNA fragmentation and electron microscopic studies

  • Published:
Medical Electron Microscopy Aims and scope Submit manuscript

Abstract

The reduced enamel epithelium transforms into a stratified squamous epithelium, i.e. a junctional epithelium, as the tooth erupts. In this study, we observed apoptosis in the reduced enamel epithelia of rats just after tooth eruption and before complete junctional epithelium formation, by the TUNEL method and electron microscopy. TUNEL-positive reactions were scattered in the reduced ameloblasts and in the external cells of the reduced enamel epithelium. Electron microscopic observation confirmed features of apoptosis, such as nuclei with chromatin condensation, cell shrinkage, and phagocytosis of apoptotic bodies by macro-phage-like cells and epithelial cells. These results suggest that apoptotic cell death is involved in the disappearance of reduced ameloblasts and the external cells of the reduced enamel epithelium during the formation of the junctional epithelium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Schroeder, H.E. andListgarten, M.A.: Fine structure of the developing epithelial attachment of human teeth.In: Monographs in Developmental Biology Vol. 2 (Wolsky, A. andTarrytown, N.Y. ed.), p. 15–81, Karger Verlag, Basal, 1977.

    Google Scholar 

  2. Abiko, Y., Kanno, H., Arai, J., Nishimura, M., Saito, M. andKaku, T.: Apoptotic cell death at tooth eruption in rat molar observed byin situ labeling of nuclear DNA fragmentation.Jpn. J. Oral. Biol. 37, 488–492 (1995).

    Google Scholar 

  3. Gavrieli, Y., Sherman, Y. andBen-Sassa, S.A.: Identification of programmed cell deathin situ via specific labeling of nuclear DNA fragmentation.J. Cell Biol. 119, 493–501 (1992).

    Article  CAS  PubMed  Google Scholar 

  4. Majino, G. andJoris, I.: Apoptosis, oncosis, and necrosis. An overview of cell death.Am. J. Pathol. 146, 3–15 (1995).

    Google Scholar 

  5. Shibata, S., Suzuki, S., Tengan, T. andYamashita, Y.: A histochemical study of apoptosis in the reduced ameloblasts of erupting mouse molars.Arch. Oral Biol. 40: 677–680 (1995).

    CAS  PubMed  Google Scholar 

  6. Lange, D. andSchroeder, H.E.: Cytochemistry and ultrastructure of gingival sulcus cells.Helv. Odotol. Acta,15, 65–86 (1971).

    CAS  Google Scholar 

  7. Ghadially, F.N.: Diagnostic Electron Microscopy of Tumours. 2nd ed., p. 326–333 Butterworth, London, 1985.

    Google Scholar 

  8. Hume, W.J.: Perspectives on Mammalian Cell Death (Potten, C.S. ed.), p. 73–79. Oxford University Press, Oxford, 1987.

    Google Scholar 

  9. Glaso, M., Sandiv, K.U. andHaaskjold, E.: Apoptosis in the rat corneal epithelium during regeneration.APMIS,101, 914–922 (1993).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abiko, Y., Nishimura, M., Arai, J. et al. Apoptosis in the reduced enamel epithelium just after tooth emergence in rats. Med Electron Microsc 29, 84–89 (1996). https://doi.org/10.1007/BF02349044

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02349044

Key words

Navigation