Antibody-dependent cell-mediated cytotoxicity against cell lines generated by liver-specific idiotype-bearing antibody
- 20 Downloads
We produced a murine monoclonal antibody (mAb), designated H2-mAb, against a fractionated soluble phase of human liver homogenate which antibody reacted with human liver cells. A human antibody possessing the same idiotype as the H2-mAb, designated LSIA (liver-specific idiotype-bearing antibody), can be measured by a sandwich enzyme-linked immunosorbent assay, using the anti-H2 idiotype antibody. The serum level of LSIA in patients with histologically proven chronic hepatitis (CH) was significantly higher than that in healthy subjects and it was also higher than that in subjects with other diseases, including systemic lupus erythematosus. In a comparison between patients with CH type B and those with CH type C, there was no significant difference in serum levels of LSIA. It was possible to purify LSIA from the sera of patients with CH. The purified LSIA bound to the human cell lines Chang and HCC-M, derived from liver cells and a hepatoma respectively, but not to HeLa cells, a uterine carcinoma derivative. The reactivity of this mAb to HCC-M was weaker than that to Change. Moreover, the presence of LSIA caused an antibody-dependent cell-mediated cytotoxic challenge against Change cells in vitro.
Key wordsmonoclonal antibody liver-specific idiotype-bearing antibody chronic hepatitis cell lines
Unable to display preview. Download preview PDF.
- 3.Morizane T, Tsuchimoto K, Ino S, et al. Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes (in Japanese with English abstract). Kanzou (Acta Hepatol Jap) 1986;27:726–735.Google Scholar
- 4.Iino A. Expression of a human liver-specific antigen defined by the murine monoclonal antibody H2 in various cell (in Japanese with English abstract). Keio Igaku (J Keio Med Soc) 1986;63:533–543.Google Scholar
- 5.Saito H, Morizane T, Iino A, et al. Characterization of human liver-specific antigen-1 (HLSA1) defined by the murine monoclonal antibody H2 (in Japanese with English abstract). Nihon Shoukakibyou Gakkai Zasshi (Jpn J Gastroenterol) 1987;84: 675–683.Google Scholar
- 6.Saito H, Morizane T, Tsuchimoto K, et al. Liver-specific idiotype-bearing antibody in autoimmune hepatitis. In: Tsuchiya M, Nagura H, Hibi T, Maro I (eds) Frontiers of Mucosal Immunology, vol 2. Amsterdam: Excerpta Medica, 1991;65–68.Google Scholar
- 10.Iwabuchi N, Morizane T, Kagawa T, et al. Study of in vitro production of the antibody bearing a liver-specific idiotype in patients with chronic hepatitis. Gastroenterology 1988;94: A551.Google Scholar
- 14.Inagaki Y, Morizane T, Matsumura S, et al. Detection of antibodies to Chang liver cells in sera from patients with chronic liver diseases by125I-labelled protein A binding assay and the effect of prednisolone and 6-mercaptopurine treatment on the level of the antibodies. Clin Exp Immunol 1984;57:79–84.PubMedGoogle Scholar
- 15.Kawanishi H. In vitro studies on IgG-mediated lymphocyte cytotoxicity in chronic active liver disease. Gastroenterology 1997;73:549–555.Google Scholar
- 18.Zeniya M, Katoh Y, Takahashi H, et al. Study of the significance of antibody-dependent cell-mediated cytotoxicity (ADCC) in chronic active hepatitis. Jpn J Gastroenterol 1981; 78:1399–1403.Google Scholar
- 19.Meyer zum Büshenfelde KH, Miesher PA. Liver-specific antigen. Purification and characterization. Clin Exp Immunol 1972;10:89–102.Google Scholar
- 23.Penna A, Chisari FV, Bertoletti A, et al. Cytotoxic T lymphocytes recognize an HLA-A2 restricted epitope within the hepatitis B virus nucleocapsid antigen. J Exp Med 1991;74: 1565–1570.Google Scholar