Journal of Gastroenterology

, Volume 30, Issue 2, pp 201–208 | Cite as

Antibody-dependent cell-mediated cytotoxicity against cell lines generated by liver-specific idiotype-bearing antibody

  • Tatehiro Kagawa
  • Hidetsugu Saito
  • Toshio Morizane
  • Kanji Tsuchimoto
  • Yasutaka Inagaki
  • Kentaro Sawaguchi
  • Naoto Iwabuchi
  • Naoki Kumagai
  • Masaharu Tsuchiya
  • Hiromasa Ishii
Liver, Pancreas, and Biliary Tract

Abstract

We produced a murine monoclonal antibody (mAb), designated H2-mAb, against a fractionated soluble phase of human liver homogenate which antibody reacted with human liver cells. A human antibody possessing the same idiotype as the H2-mAb, designated LSIA (liver-specific idiotype-bearing antibody), can be measured by a sandwich enzyme-linked immunosorbent assay, using the anti-H2 idiotype antibody. The serum level of LSIA in patients with histologically proven chronic hepatitis (CH) was significantly higher than that in healthy subjects and it was also higher than that in subjects with other diseases, including systemic lupus erythematosus. In a comparison between patients with CH type B and those with CH type C, there was no significant difference in serum levels of LSIA. It was possible to purify LSIA from the sera of patients with CH. The purified LSIA bound to the human cell lines Chang and HCC-M, derived from liver cells and a hepatoma respectively, but not to HeLa cells, a uterine carcinoma derivative. The reactivity of this mAb to HCC-M was weaker than that to Change. Moreover, the presence of LSIA caused an antibody-dependent cell-mediated cytotoxic challenge against Change cells in vitro.

Key words

monoclonal antibody liver-specific idiotype-bearing antibody chronic hepatitis cell lines 

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References

  1. 1.
    Storch W. Immunopathology and humoral autoimmunity in chronic active hepatitis. Scand J Gastroenterol 1988;23: 513–516.PubMedGoogle Scholar
  2. 2.
    Vento S, Eddleston A. Autoimmunity and liver diseases. Prog Liver Dis 1990;9:335–343.PubMedGoogle Scholar
  3. 3.
    Morizane T, Tsuchimoto K, Ino S, et al. Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes (in Japanese with English abstract). Kanzou (Acta Hepatol Jap) 1986;27:726–735.Google Scholar
  4. 4.
    Iino A. Expression of a human liver-specific antigen defined by the murine monoclonal antibody H2 in various cell (in Japanese with English abstract). Keio Igaku (J Keio Med Soc) 1986;63:533–543.Google Scholar
  5. 5.
    Saito H, Morizane T, Iino A, et al. Characterization of human liver-specific antigen-1 (HLSA1) defined by the murine monoclonal antibody H2 (in Japanese with English abstract). Nihon Shoukakibyou Gakkai Zasshi (Jpn J Gastroenterol) 1987;84: 675–683.Google Scholar
  6. 6.
    Saito H, Morizane T, Tsuchimoto K, et al. Liver-specific idiotype-bearing antibody in autoimmune hepatitis. In: Tsuchiya M, Nagura H, Hibi T, Maro I (eds) Frontiers of Mucosal Immunology, vol 2. Amsterdam: Excerpta Medica, 1991;65–68.Google Scholar
  7. 7.
    Chang RSM. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc Soc Exp Biol Med 1954;87: 440–443.PubMedGoogle Scholar
  8. 8.
    Watanabe T, Morizane T, Tsuchimoto K, et al. Establishment of a cell line (HCC-M) from a human hepatocellular carcinoma. Int J Cancer 1983;32:141–146.PubMedGoogle Scholar
  9. 9.
    Saito H, Morizane T, Watanabe T, et al. Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T, and PLC/PRF/5. Keio J Med 1991;40:139–145.PubMedGoogle Scholar
  10. 10.
    Iwabuchi N, Morizane T, Kagawa T, et al. Study of in vitro production of the antibody bearing a liver-specific idiotype in patients with chronic hepatitis. Gastroenterology 1988;94: A551.Google Scholar
  11. 11.
    Saito H, Morizane T, Watanabe T, et al. Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T. Int J Cancer 1991;48:291–296.PubMedGoogle Scholar
  12. 12.
    Saito H, Kagawa T, Tada S, et al. Effect of dexamethasone, dimethylsulfoxide and sodium butyrate on a human hepatoma cell line PLC/PRF/5. Cancer Biochem Biophys 1992;13:75–84.PubMedGoogle Scholar
  13. 13.
    Saito H, Tada S, Ebinuma H, et al. Changes of antigen expression on human hepatoma cell lines caused by sodium butyrate, a differentation inducer. J Gastroenterol 1994;29:733–739.PubMedGoogle Scholar
  14. 14.
    Inagaki Y, Morizane T, Matsumura S, et al. Detection of antibodies to Chang liver cells in sera from patients with chronic liver diseases by125I-labelled protein A binding assay and the effect of prednisolone and 6-mercaptopurine treatment on the level of the antibodies. Clin Exp Immunol 1984;57:79–84.PubMedGoogle Scholar
  15. 15.
    Kawanishi H. In vitro studies on IgG-mediated lymphocyte cytotoxicity in chronic active liver disease. Gastroenterology 1997;73:549–555.Google Scholar
  16. 16.
    Vergani D, Mieli-Vergani G, Mondelli M, et al. Immunoglobulin on the surface of isolated hepatocytes is associated with antibody-dependent cell-mediated cytotoxicity and liver damage. Liver 1987;7:307–315.PubMedGoogle Scholar
  17. 17.
    Cochrane AMG, Moussouros A, Portmann B, et al. Lymphocyte cytotoxicity for isolated hepatocytes in alcoholic liver disease. Gastroenterology 1977;72:918–923.PubMedGoogle Scholar
  18. 18.
    Zeniya M, Katoh Y, Takahashi H, et al. Study of the significance of antibody-dependent cell-mediated cytotoxicity (ADCC) in chronic active hepatitis. Jpn J Gastroenterol 1981; 78:1399–1403.Google Scholar
  19. 19.
    Meyer zum Büshenfelde KH, Miesher PA. Liver-specific antigen. Purification and characterization. Clin Exp Immunol 1972;10:89–102.Google Scholar
  20. 20.
    Guilhot S, Fowler P, Portillo G, et al. Hepatitis B virus (HBV)-specific cytotoxic T-cell response in humans: Production of target cells by stable expression of HBV-encoded proteins in immortalized human B-cell lines. J Virol 1992;66:2670–2678.PubMedGoogle Scholar
  21. 21.
    Bertoletti A, Ferrari C, Fiaccadori F, et al. HLA class I restrited human cytotoxic T cells recognize endogenously synthesized hepatitis B virus nucleocapsid antigen. Proc Natl Acad Sci USA 1991;88:10445–10449.PubMedGoogle Scholar
  22. 22.
    Mondelli MU, Bortolotti F, Potisso P, et al. Definition of hepatitis B virus (HBV)-specific target antigens recognized by cytotoxic T cells in acute HBV infection. Clin Exp Immunol 1987;68:242–250.PubMedGoogle Scholar
  23. 23.
    Penna A, Chisari FV, Bertoletti A, et al. Cytotoxic T lymphocytes recognize an HLA-A2 restricted epitope within the hepatitis B virus nucleocapsid antigen. J Exp Med 1991;74: 1565–1570.Google Scholar
  24. 24.
    Milich DR, Hughes JL, Houghten R, et al. Functional identification of agretopic and epitopic residues within an HBcAg T cell determinant. J Immunol 1989;143:3141–3147.PubMedGoogle Scholar
  25. 25.
    Moriyama T, Guilhot S, Klopchin K, et al. Immunobiology and pathogenesis of hepatocellular injury in hepatitis B virus transgenic mice. Science 1990;248:361–364.PubMedGoogle Scholar
  26. 26.
    Baraldini M, Miglio F, Cursaro C, et al. Clinical significance of antibodies to polymerized human albumin detected by enzymelinked immunosorbent assay. Ric Clin Lab 1988;18:291–300.PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Tatehiro Kagawa
    • 1
  • Hidetsugu Saito
    • 1
  • Toshio Morizane
    • 2
  • Kanji Tsuchimoto
    • 3
  • Yasutaka Inagaki
    • 4
  • Kentaro Sawaguchi
    • 5
  • Naoto Iwabuchi
    • 3
  • Naoki Kumagai
    • 3
  • Masaharu Tsuchiya
    • 1
  • Hiromasa Ishii
    • 1
  1. 1.Department of Internal Medicine, School of MedicineKeio UniversityTokyoJapan
  2. 2.Department of Internal MedicineKanagawa Dental CollegeYokosukaJapan
  3. 3.Department of Internal MedicineKitasato Institute HospitalTokyoJapan
  4. 4.Department of Internal MedicineNihon Kohkan HospitalKawasakiJapan
  5. 5.Department of Internal MedicineNational Tochigi HospitalUtsunomiyaJapan

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