Journal of Gastroenterology

, Volume 30, Issue 2, pp 135–141 | Cite as

Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells

  • Yoshikazu Kinoshita
  • Sazzad Hassan
  • Hirohisa Nakata
  • Masakyo Asahara
  • Yumi Matsushima
  • Chiharu Kawanami
  • Cai Yao Ping
  • Ding Min
  • Akira Nakamura
  • Tsutomu Chiba
Alimentary Tract

Abstract

Proliferating cells in the gastric mucosal epithelium were successfully enriched by counterflow elutriation in a medium-sized cell fraction. When inoculated on culture plates coated with E-C-L cell attachment matrix, these cells differentiated into mucus-producing cells after reaching confluence. Northern blot analysis did not detect any transcript of the proton pump, histidine decarboxylase, somatostatin, or pepsinogen I, indicating the absence of parietal, ECL, D, and chief cells in the confluent monolayer. These mucus-producing cell monolayers that respond to various growth factors may be a suitable model with which to investigate the function of gastric mucus cells in vitro.

Key words

stomach isolated cell cell culture growth factors 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Yoshikazu Kinoshita
    • 1
  • Sazzad Hassan
    • 1
  • Hirohisa Nakata
    • 1
  • Masakyo Asahara
    • 1
  • Yumi Matsushima
    • 1
  • Chiharu Kawanami
    • 1
  • Cai Yao Ping
    • 1
  • Ding Min
    • 1
  • Akira Nakamura
    • 1
  • Tsutomu Chiba
    • 1
  1. 1.Division of Gerontology, Department of MedicineKobe University School of MedicineKobeJapan

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