A scanning electron microscopic study of the three-dimensional configuration of isolated crypts from human colon adenomas
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The three-dimensional configurations of isolated crypts from 10 normal human colons and of adenomatous crypts from 23 tubular adenomas of the colon (diameters, 2 to 30 mm) were investigated by scanning electron microscopy (SEM) after the removal of extracellular material with 8N HCl. The isolated, normal colonic crypts were simple tubular glands, about 50 μm in diameter and 300–400 μm in length. In the smaller adenomas, the adenomatous crypts ranged in length from 700 to 1,400 μm and exhibited multiple tubular or tuberous branches, as well as bending, meandering and twisting. The diameters of the branching tubules were 30–100 μm. In the crypts of the larger adenomas, additional features were noted, including marked flaring at the apical portion (300–700 μm in width) and elongation of the crypts (500–2,000 μm in length).
Key wordsHuman colon Colonic adenoma Removal of extracellular material Scanning electron microscopy
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