Medical Electron Microscopy

, Volume 27, Issue 1, pp 55–60 | Cite as

A scanning electron microscopic study of the three-dimensional configuration of isolated crypts from human colon adenomas

  • Keijiro Araki
  • Michiya Kobayashi
  • Takuro Ogata
Short Report


The three-dimensional configurations of isolated crypts from 10 normal human colons and of adenomatous crypts from 23 tubular adenomas of the colon (diameters, 2 to 30 mm) were investigated by scanning electron microscopy (SEM) after the removal of extracellular material with 8N HCl. The isolated, normal colonic crypts were simple tubular glands, about 50 μm in diameter and 300–400 μm in length. In the smaller adenomas, the adenomatous crypts ranged in length from 700 to 1,400 μm and exhibited multiple tubular or tuberous branches, as well as bending, meandering and twisting. The diameters of the branching tubules were 30–100 μm. In the crypts of the larger adenomas, additional features were noted, including marked flaring at the apical portion (300–700 μm in width) and elongation of the crypts (500–2,000 μm in length).

Key words

Human colon Colonic adenoma Removal of extracellular material Scanning electron microscopy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1).
    Uehara, Y: Methods to disclose non-luminal surfaces of tissues and cells for SEM examination (Japanese text).Electron Microsc. 16, 40–46 (1981).Google Scholar
  2. 2).
    Evan, A.P., Dail, W.G., Dammrose, D. andPalmer, C.: Scanning electron microscopy of cell surfaces following removal of extracellular material.Anat. Rec. 185, 433–446 (1976).CrossRefPubMedGoogle Scholar
  3. 3).
    Mitsushima, A andInoue, T. Scanning electron microscopic observation of small-sized specimens obtained by an NaOH digestion method (Japanese text).Proc. 49th Annu. Meet. Jpn. Soc. Electron Microsc. 26-I-1030, 22 (1993).Google Scholar
  4. 4).
    Fenoglio-Preiser, C.M., Pascal, R. andPerzin, K.H.: Adenomas of the colon and rectum.In: Tumors of the Intestines, Fascicle 27, Atlas of Tumor Pathology (Hartmann, W.H. ed.), p. 76–150, Armed Forces Institute of Pathology, Washington, D.C., 1990.Google Scholar
  5. 5).
    Kaye, G.I., Pascal, R.R. andLane, N.: The colonic pericryptal fibroblast sheath: replication, migration and cytodifferentiation of a mesenchymal cell system in adult tissue. III.Gastroenterology,60, 515–536 (1971).PubMedGoogle Scholar

Copyright information

© The Clinical Electron Microscopy Society of Japan 1994

Authors and Affiliations

  • Keijiro Araki
    • 1
  • Michiya Kobayashi
    • 1
  • Takuro Ogata
    • 1
  1. 1.The First Department of SurgeryKochi Medical SchoolNankokuJapan

Personalised recommendations