Abstract
An ultrastructural investigation was made of the distribution of immune complexes (IC) and anionic sites using cationic colloidal gold in the glomerular basement membrane (GBM) of kidneys with murine graft-versus-host disease. At 8 weeks, a decrease in the number of anionic sites around deposits of IC in the GBM was noted. These anionic sites could be removed by treatment with hyaluronidase or chondroitinase, but not with heparitinase, suggesting that they consisted mainly of hyaluronic acid. These results support the theory that IC deposits are associated with a decrease in the number of GBM anionic sites which mainly consist of hyaluronic acid.
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Kitazawa, K., Tsumuraya, T., Shioda, S. et al. Detection of hyaluronic acid in the glomerular basement membrane of murine chronic graft-versus-host disease. Med Electron Microsc 27, 165–167 (1994). https://doi.org/10.1007/BF02348182
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DOI: https://doi.org/10.1007/BF02348182