Medical Electron Microscopy

, Volume 29, Issue 1, pp 23–27 | Cite as

Endothelin-1 (ET-1) enhances bile canalicular contractions in cultured rat hepatocytes

A time lapse cinematographic and electron microscopic study
  • Hiroaki Yokomori
  • Masaya Oda
  • Jing-Y Han
  • Mariko Ogi
  • Yoshitaka Kamegaya
  • Nobuhiro Tsukada
  • Yasutada Akiba
  • Masahiko Nakamura
  • Tadashi Motoori
  • Hiromasa Ishii
Article
  • 24 Downloads

Abstract

Time lapse cinematographic analysis revealed that the frequency of canalicular contractions in primary monolayer-cultured hepatocytes (couples and triplets) isolated from a rat liver was increased by endothelin-1 (ET-1)-treatment as compared with that of a control group. By transmission electron microscopy, vesicles were more frequently observed to be opened to the canalicular lumens during the contracting stage of the bile canaliculi in the ET-1-treated couplets and triplets than in the control group.

Key words

Bile canalicular contraction Endothelin-1 Primary cultured hepatocyte 

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Copyright information

© The Clinical Electron Microscopy Society of Japan 1996

Authors and Affiliations

  • Hiroaki Yokomori
    • 1
  • Masaya Oda
    • 3
  • Jing-Y Han
    • 3
  • Mariko Ogi
    • 2
  • Yoshitaka Kamegaya
    • 3
  • Nobuhiro Tsukada
    • 3
  • Yasutada Akiba
    • 3
  • Masahiko Nakamura
    • 3
  • Tadashi Motoori
    • 2
  • Hiromasa Ishii
    • 3
  1. 1.Department of Internal MedicineKitasato Institute Midical Center HospitalKitamotoJapan
  2. 2.Laboratory of PathologyKitasato Institute Medical Center HospitalKitamotoJapan
  3. 3.Department of Internal MedicineSchool of Medicine, Keio UniversityTokyoJapan

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