Advertisement

Medical Electron Microscopy

, Volume 28, Issue 2, pp 80–87 | Cite as

Morphological changes in the inner ear induced by bacterial endotoxin

  • Kensuke Watanabe
  • Nagae Naito
  • Yasuo Tanaka
Article

Abstract

Escherichia coli-derived endotoxin was introduced into the middle ears of 10 guinea pigs 24 hr after intraperitoneal injection of the same endotoxin. The cochleae were removed 24 hr after the introduction of the endotoxin into the middle ears and observed by light and electron microscopy. Inflammatory cell infitration and bleeding were observed in both endolymphatic and perilymphatic spaces. Inflammatory cells also infiltrated the stria vascularis, spiral ligament, spiral prominence and the organ of Corti. Blood sludge was observed in almost all of the strial capillaries of the upper three turns, but in few of the basal turns. The hair cells were intact in every turn except for a small number of degenerated mitochondria. Intermediate cells of the stria vascularis were shrunken, and some of them showed degenerative changes. Marginal cells had also shrunken, but they were less shrunken than the intermediate cells. The shrinking of both cells caused the enlargement of the intercellular space of the stria vascularis.

Key words

Endotoxin Inner ear Electron microscopy Inflammatory cell Stria vascularis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    DeMaria, T.F., Prior, R.B., Briggs, B.R., Lim, D.J. andBirck, H.G.: Endotoxin in middle-ear effusions from patients with chronic otitis media with effusion.J. Clin. Microbiol. 20, 15–17 (1984).PubMedGoogle Scholar
  2. 2).
    Iino, Y., Kaneko, Y. andTakasaka, T.: Endotoxin in middle ear effusions tested with Limulus assay.Acta Otolaryngol. 100, 42–50 (1985).PubMedGoogle Scholar
  3. 3).
    Sakakura, K., Hamaguchi, Y., Harada, T., Yamagiwa, M. andSakakura, Y.: Endotoxin and lysosomal protease activity in acute and chronic otitis media with effusion.Ann. Otol. Rhinol. Laryngol. 99, 379–385 (1990).PubMedGoogle Scholar
  4. 4).
    Kawauchi, H., DeMaria, T.F. andLim, D.J.: Endotoxin permeability through the round window.Acta Otolaryngol. (Stockh.), suppl.457, 100–115 (1988).Google Scholar
  5. 5).
    Lim, D.J., Kawauchi, H. andDeMaria, T.F.: Role of middle ear endotoxin in inner ear inflammatory response and hydrops: long-term study.Ann. Otol. Rhinol. Laryngol. 99, 33–34 (1988).Google Scholar
  6. 6).
    Nakai, Y., Morimoto, A., Chang, K.C., Yamada, M. andNishisato, K.: Inner ear damage induced by bacterial endotoxin.Arch. Otorhinolaryngol. 229, 209–220 (1980).PubMedGoogle Scholar
  7. 7).
    Watanabe, K. andKomatsuzaki, A.: Membranous cytoplasmic bodies in the lateral wall of the cat cochlea.Ann. Otol. Rhinol. Laryngol. 101, 355–359 (1992).PubMedGoogle Scholar
  8. 8).
    Watanabe, K., Yokoyama, K. andHarada, H.: Changes in capillaries of the strial vascularis after hemorrhagic shock.Ann. Otol. Rhinol. Laryngol. 99, 660–665 (1990).PubMedGoogle Scholar
  9. 9).
    Watanabe, K.: Change in the capillary permeability of the strial vascularis by different methods of death and fixation.Ann. Otol. Rhinol Laryngol. 95, 427–431 (1986).PubMedGoogle Scholar
  10. 10).
    Watanabe, K.: The characteristics of the strial capillaries in each turn of the cochlea.Pract. Otol. (Kyoto),81, 887–893 (1988).Google Scholar
  11. 11).
    Morrison, D.C. andUlevitch, R.J.: The effects of bacterial endotoxins on host mediation systems.Am. J. Pathol. 93, 527–617 (1978).Google Scholar
  12. 12).
    Paparella, M.M., Oda, M., Hiraide, F. andBrady, D.: Pathology of sensorineural hearing loss in otitis media.Ann. Otol. Rhinol. Laryngol. 81, 632–647 (1972).PubMedGoogle Scholar
  13. 13).
    Shwartzman, G.: A new phenomenon of local skin reactivity to B typhosus culture filtrate.Proc. Soc. Exp. Biol. Med. 25, 560–561 (1928).Google Scholar
  14. 14).
    Shwartzman, G.: Studies onBacillus typhosus toxic substances. I. Phenomenon of local skin reactivity to B typhosus culture filtrate.J. Exp. Med. 48, 247–268 (1928).CrossRefGoogle Scholar
  15. 15).
    Apitz, K.: A study of the generalized shwartzman phenomenon.J. Immunol. 29, 255–266 (1935).Google Scholar
  16. 16).
    Tarlow, M.J., Comis, S.D. andOsborne, M.P.: Endotoxin induced damage to the cochlea in guinea pigs.Arch. Dis. Child. 66, 181–184 (1991).PubMedGoogle Scholar
  17. 17).
    Anniko, M., Hellström, S., Schmidt, S.H. andSpandow, O.: Toxic effects on inner ear of noxious agents passing through the round window membrane.Acta Otolaryngol. (Stockh.), suppl.457, 49–56 (1988).Google Scholar
  18. 18).
    Spandow, O., Anniko, M. andHellström, S.: Inner ear disturbances following inoculation of endotoxin into the middle ear.Acta Otolaryngol. (Stockh.),107, 90–96 (1989).Google Scholar

Copyright information

© The Clinical Electron Microscopy Society of Japan 1995

Authors and Affiliations

  • Kensuke Watanabe
    • 1
  • Nagae Naito
    • 1
  • Yasuo Tanaka
    • 1
  1. 1.Department of Otorhinolaryngology, Koshigaya HospitalDokkyo University School of MedicineKoshigayaJapan

Personalised recommendations