Abstract
Tubular injury leading to tubular atrophy and tubular loss is one of the characteristic features of chronic renal failure. To reveal the mechanism of tubular atrophy in chronic renal failure, the involvement of apoptosis was studied byin situ nick end labeling of biotinylated deoxyuridine by terminal deoxy nucleotidyl transferase (TUNEL) as well as by electron microscopy. TUNEL-positive cells and heterophagosomes containing apoptotic body-like structures having nuclear fragments with condensed chromatin were observed in the renal tubular epithelium 8 weeks after nephrectomy. It has become apparent from our study that atrophy of the tubules in chronic renal failure results, to some extent, from cell deletion by apoptosis.
This is a preview of subscription content, log in via an institution to check access.
References
Rennke, H.G., Anderson, S. andBrenner, B.M.: Structural and functional correlations in the progression of kidney diseases.In: Renal Pathology vol. I (Tischer, C.C. andBrenner, B.M. ed.) p. 43966, Lippincott Co., New York, 1989.
Kerr, J.F.R.: Apoptosis: a basic biological phenomenon with wide range implications in tissue kinetics.Br. J. Cancer,26, 239–257 (1972).
Cohen, J.J.: Apoptosis.Immunol. Today,14, 126–130 (1993).
Duvall, E. andWyllie, A.H.: Death and cell.Immunol. Today,7, 115–119 (1986).
Hostetter, T.H., Olson, J.L., Venkatachalam, M.A. andBrenner, B.M.: Hyperfiltration in remnant nephrons: a potentially adverse response to renal ablation.Am. J. Physiol. 241, F85-F93 (1981).
Makino, H., Theng, L.Z., Kumagai, I., Takatori, K., Ota, Z. andYoshifusa, H.: Progressive glomerular sclerosis and renal failure in rat with 7/8 nephrectomy model.Jpn. J. Nephrol. 32, 127–136 (1990).
Gavrieli, Y., Sherman, Y. andBen-Sasson, S.A.: Identification of programmed cell deathin situ via specific labeling of nuclear DNA fragmentation.J. Cell. Biol. 119, 493–501 (1992).
Makino, H., Yamasaki, Y., Haramoto, T., Shikata, K., Hironaka, K., Ota, Z. andKanwar, Y.S.: Ultrastructural changes of extracellular matrices in diabetic nephropathy revealed by high resolution scanning and immunoelectron microscopy.Lab. Invest. 68, 45–55 (1993).
Gobe, G.C. andAxelsen, R.A.: Genesis of renal tubular atrophy in experimental hydronephrosis in the rat.Lab. Invest. 56, 273–281 (1987).
Gobe, G., Axelsen, R.A. andSearle, J.W.: Cellular events in experimental unilateral ischemic renal atrophy and regeneration after contralateral nephrectomy.Lab. Invest. 63, 770–779 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Makino, H., Sugiyama, H., Kashihara, N. et al. Apoptosis of tubular epithelial cells in rats with chronic renal failure. Med Electron Microsc 28, 210–212 (1995). https://doi.org/10.1007/BF02347965
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02347965