Journal of Infection and Chemotherapy

, Volume 1, Issue 1, pp 40–49 | Cite as

A retrospective study of methicillin-resistantStaphylococcus aureus clinical strains in Tokyo university hospital

  • Tae Tanaka
  • Katsuko Okuzumi
  • Aikichi Iwamoto
  • Keiichi Hiramatsu
Original Articles


Staphylococcus aureus clinical strains isolated in 1982 and 1992 at Tokyo University Hospital were studied for their carriage ofmecA gene, antibiotic susceptibility patterns, toxin production, coagulase isotypes, and ribotyping patterns. ThemecA-carrying strains in 1982 were mostly producers of type-4 coagulase (21 out of 31;68%), but producers of other coagulase types (type-1,-2,-7) were also found. The degree of methicillin resistance varied but was moderate with these strains (MIC50=16; range, 0.5–512 μg/ml at 32°C with 2% NaCl), and some strains were even judged to be susceptible to methicillin in spite of their carriage ofmecA gene. In comparison,mecA-carrying strains in 1992 were mostly of single type in terms of production of type 2 coagulase (26 out of 27;96%), and high-level methicillin resistance (MIC50>-512). Clonal expansion of the coagulase type-2 strain was implicated under selective pressure of antibiotic usage during the last decade.

Key words

methicillin resistance Staphylococcus aureus imipenem, coagulase type 


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Copyright information

© Japan Society of Chemotherapy/Churchill Livingstone Japan 1995

Authors and Affiliations

  • Tae Tanaka
    • 3
  • Katsuko Okuzumi
    • 1
  • Aikichi Iwamoto
    • 2
  • Keiichi Hiramatsu
    • 3
  1. 1.Laboratory for Clinical MicrobiologyTokyo University HospitalTokyo
  2. 2.Department of Infectious Diseases, Institute of Medical ScienceUniversity of TokyoTokyo
  3. 3.Department of BacteriologyJuntendo UniversityTokyoJapan

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