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Use of acetylcholine (Ach) for spreading metaphase chromosomes and application to the cytogenetic analysis of human stomach cancers

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Abstract

Cytogenetic studies of biopsy specimens endoscopically obtained from gastric cancers were performed in our laboratory. The necessity for well-spread chromosomes for analysis has resulted in the development of a new technique, in which culture medium containing acetylcholine (Ach) is used; with this new technique, the number of metaphases of analyzable karyotypes was significantly increased (P<0.01 compared with a previous method in which Ach was not used). The mean ratio of metaphase numbers of analyzable karyotypes in four cases in which Ach was used was 38.1±8.1%, a value more than four times the number in the seven cases in which Ach was not used. It is possible that Ach may decrease the viscosity of the cytoplasm in gastric cancer cells by disrupting microfilaments.

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Authors and Affiliations

  1. Department of Radiology, Yamaguchi University, School of Medicine, 1144 Kogushi, 755, Ube, Yamaguchi, Japan

    Kazuhiko Sasai, Taishi Nakada, Rikuo Tanaka, Mitsutoshi Kawamura & Takashi Nakanishi

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  1. Kazuhiko Sasai
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  2. Taishi Nakada
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  3. Rikuo Tanaka
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  4. Mitsutoshi Kawamura
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  5. Takashi Nakanishi
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Sasai, K., Nakada, T., Tanaka, R. et al. Use of acetylcholine (Ach) for spreading metaphase chromosomes and application to the cytogenetic analysis of human stomach cancers. J Gastroenterol 30, 310–314 (1995). https://doi.org/10.1007/BF02347504

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  • DOI: https://doi.org/10.1007/BF02347504

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