Journal of Gastroenterology

, Volume 30, Issue 3, pp 295–300 | Cite as

Serum antibody againstHelicobacter pylori assayed by a new capture ELISA

  • Hiroya Nakata
  • Hidekazu Itoh
  • Yukihiro Yokoya
  • Jun Kawai
  • Shingo Nishioka
  • Kazuaki Miyamoto
  • Noritoshi Kitamoto
  • Hiroyuki Miyamoto
  • Tomoyuki Tanaka
Alimentary Tract

Abstract

We developed a highly specific detection technique for serum antibody, using a monoclonal antibody to a specific antigen ofHelicobacter pylori. A monoclonal antibody preparation that reacted with the 54-kDa molecule ofH. pylori antigens was obtained. Using this preparation, an antigen-capture enzymelinked immunosorbent assay (ELISA) was established by fixation of the monoclonal antibody, followed by reaction with sonicated whole cell antigens. The serum antibody titers of patients with gastritis and peptic ulcers were significantly higher than control titers, and the antibody titer correlated with the histological severity of gastritis. Patients positive forH. pylori by bacterial culture had higher titers thanH. pylori-negative patients. Our new ELISA may be useful for the diagnosis ofH. pylori infections and for evaluation of the severity of gastric inflammation.

Key words

Helicobacter pylori enzyme-linked immunosorbent assay monoclonal antibody 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Hiroya Nakata
    • 1
  • Hidekazu Itoh
    • 1
  • Yukihiro Yokoya
    • 1
  • Jun Kawai
    • 1
  • Shingo Nishioka
    • 1
  • Kazuaki Miyamoto
    • 2
  • Noritoshi Kitamoto
    • 2
  • Hiroyuki Miyamoto
    • 2
  • Tomoyuki Tanaka
    • 3
  1. 1.Second Department of Internal MedicineWakayama Medical CollegeWakayamaJapan
  2. 2.Department of MicrobiologyWakayama Medical CollegeWakayamaJapan
  3. 3.Kinan General HospitalTanabeJapan

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