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In vivo iontophoretic delivery of calcium ions through guinea pig skin enhanced by direct and pulsating current

  • Zs. Szántó
  • L. Papp
  • J. Kónya
  • N. Nagy
  • Zs. Lengyel
Article

Abstract

Studies were initiated to investigate the effect of the delivery mode of45Ca ions through guinea pig skin in vivo. Direct current (DC), pulsating current (PC) and a Bernard current form, the “courtes periodes” current profile (CP) were applied with the same current density (0.16 mA/cm2) and for the same duration (30 minutes). The45Ca ions were delivered from a Ca-bentonite patch radiolabeled with45Ca (a natural mineral clay rich in calcium, 50 mgCa/g). The total quantity of applied bentonite was 1.5g×10 days=15g.45Ca was counted in different biological samples of the animals. The delivery of45Ca ions into the body (systemic effect) is the highest when CP current is applied (6.87±0.95·10−12g/samples). The local effect appears to be more effective in case of DC current mode (5.89±0.12·10−12g/0.5g bone). Total calcium measurements proved that the result of transdermal radiocalcium delivery is not only an ion exchange process at the surface of the bone but a deposition of calcium ions into the hydroxiapatite matrix (the net calcium introduction, which represent the difference between the total calcium into the treated bone and total calcium into untreated bone varied from 15.52±2.42·10−3g/0.5g bone to 44.30±3.50·10−3g/0.5g bone). The results suggest that iontophoresis could be used to accumulate calcium into different target tissues using the appropriate current system.

Keywords

Bentonite Mineral Clay Direct Current Pulsate Current Current Mode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Akadémiai Kiadó 1999

Authors and Affiliations

  • Zs. Szántó
    • 1
  • L. Papp
    • 2
  • J. Kónya
    • 3
  • N. Nagy
    • 3
  • Zs. Lengyel
    • 4
  1. 1.Institute of Nuclear Research of the Hungarian Academy of SciencesDebrecenHungary
  2. 2.Department of Inorganic and Analytical ChemistryLajos Kossuth UniversityDebrecenHungary
  3. 3.Department of Isotope ApplicationLajos Kossuth UniversityDebrecenHungary
  4. 4.PET CenterUniversity of Medical School of DebrecenDebrecenHungary

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