The structure of the salivary gland of the moth (Manduca sexta)

Summary

The salivary glands of the moth,Manduca sexta, are described, emphasizing correlations between structure and function in an attempt to explain the production of a dilute saliva. Each of the paired glands consists of five distinct regions: protein secreting, fluid secreting, thin duct, bulbous duct, and common duct. Each region constists of a single, ultrastructurally distinct, cell type. It is proposed that the protein and fluid secreting regions produce an enzyme-containing primary saliva isosmotic with the haemolymph; this saliva is modified in the remaining regions of the gland to yield a dilute saliva.

This is a preview of subscription content, log in to check access.

References

  1. Berridge, M. J., Gupta, B. L.: Fine-structural changes in relation to ion and water transport in the rectal papillae of the blowfly,Calliphora. J. Cell Sci.2, 89–112 (1967)

    CAS  PubMed  Google Scholar 

  2. Bland, K. P., House, C. R.: Function of the salivary glands of the cockroach,Nauphoeta cinerea. J. Insect Physiol.17, 2069–2084 (1971)

    Article  CAS  PubMed  Google Scholar 

  3. Copeland, D. E.: A mitochondrial pump in the cells of the anal papillae of mosquito larvae. J. Cell Biol.23, 253–263 (1964)

    Article  CAS  PubMed  Google Scholar 

  4. Dahlman, D. L.: Trehalase activity in tobacco hornworm tissue. Ann. entomol. Soc. Amer.63, 1563–1565 (1970)

    CAS  Google Scholar 

  5. Diamond, J. M.: Water-solute coupling and ion selectivity in epithelia. Phil. Trans. B262, 141–151 (1971)

    CAS  Google Scholar 

  6. Diamond, J. M., Bossert, W. H.: Standing-gradient osmotic flow. A mechanism for coupling of water and solute transport in epithelia. J. gen. Physiol.50, 2061–2083 (1967)

    Article  CAS  PubMed  Google Scholar 

  7. Kendall, M. D.: The fine structure of the salivary glands of the desert locustSchistocerca gregaria Forskâl. Z. Zellforsch.98, 399–420 (1969)

    Article  CAS  PubMed  Google Scholar 

  8. Kessel, R. G., Beams, H. W.: Electron microscope observations on the salivary gland of the cockroach,Periplaneta americana. Z. Zellforsch.59, 857–877 (1963)

    Article  CAS  PubMed  Google Scholar 

  9. Maser, M. D., Powell, T. E., Philpott, C. W.: Relationships among pH, osmolality, and concentration of fixative solutions. Stain Technol.42, 175–182 (1967)

    CAS  PubMed  Google Scholar 

  10. Oschman, J. C., Berridge, M. J.: Structural and functional aspects of salivary gland secretion in Calliphora. Tissue and Cell2, 281–310 (1970)

    Google Scholar 

  11. Oschman, J. C., Wall, B. J.: The structure of the rectal pads of Periplaneta americana L. with regard to fluid transport. J. Morph.127, 475–509 (1969)

    Google Scholar 

  12. Revel, J. P., Karnovsky, M. J.: Hexagonal array of subunits in intracellular junctions of the mouse heart and liver. J. Cell Biol.33, C7-C12 (1967)

    Article  CAS  PubMed  Google Scholar 

  13. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963)

    Article  CAS  PubMed  Google Scholar 

  14. Richardson, K. C.: Electron microscopic identification of autonomic nerve endings. Nature (Lond.)210, 756 (1966)

    CAS  Google Scholar 

  15. Robertson, H. A.: The innervation of the Salivary gland of the moth,Manduca sexta. Z. Zellforsch. (in press)

  16. Wigglesworth, V. B.: The principles of insect physiology, 6th ed. London: Methuen 1965

    Google Scholar 

  17. Yamamoto, R. T.: Mass rearing of the tobacco hornworm-1. Egg production. J. econ. Ent.61, 170–174 (1968)

    Google Scholar 

  18. Yamamoto, R. T.: Mass rearing of the tobacco hornworm-11. Larval rearing and pupation. J. econ. Ent.62, 1427–1431 (1969)

    Google Scholar 

  19. Young, J. A., Fromter, E., Schogel, E., Hamann, K. F.: Micropuncture and perfusion studies of fluid and electrolyte transport in the rat submaxillary gland. In: Secretory mechanisms of salivary glands (Schneyer and Schneyer eds.), p. 11–31. New York-London: Academic Press 1967

    Google Scholar 

Download references

Author information

Affiliations

Authors

Additional information

Acknowledgements. We thank Professor T. Weis-Fogh for accommodation in the Department of Zoology and Dr. J. E. Treherne for use of A.R.C. facilities. We are especially grateful to Dr. Nancy Lane for encouragement, advice and critical comments and to Drs. M. J. Berridge and S.H.P. Maddrell for helpful discussion. H.A.R. is grateful to Clare College, Cambridge for financial aid.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Leslie, R.A., Robertson, H.A. The structure of the salivary gland of the moth (Manduca sexta). Z.Zellforsch 146, 553–564 (1973). https://doi.org/10.1007/BF02347183

Download citation

Key words

  • Salivary gland Moth
  • Protein secretion
  • Fluid secretion
  • Electron microscopy