Advertisement

Electron microscopic study of rat pituitary primordium in organ culture

  • Yûichi G. Watanabe
  • Hideo Matsumura
  • Shigeo Daikoku
Article

Summary

To see whether adenohypophysial tissue has the capacity of self-differentiation, Rathke's pouch together with part of the neurohypophysial primordium was isolated from 12-(Group A), 14- (Group B) and 15-day-old (Group C) rat fetuses and cultivated for 9, 6 and 5 days, respectively. Electron microscopic examination of these explants showed many adenohypophysial cells containing electron dense granules, indicative of cytodifferentiation in all groups. Three cell types were distinguishable on the basis of the size of their granules. Variable amounts of neurohypophysial tissue were seen in some explants of Groups B and C, but not A. This “pars nervosa” contained pituicytes with lipid-like inclusions in their cytoplasm but no neurosecretory axons. The adenohypophysial cells abutting on the pars nervosa were arranged rather regularly like cells of the pars intermedia which contained a few granules of about 200 mμ diameter.

From these data it is concluded that in rats granule formation in the adenohypophysial primordial cells starts in the absence of hypothalamic neurosecretory substances. It is uncertain from this study whether these cells actually contain hypophysial hormones.

Key words

Hypophysis Primordium Rat Organ culture Electron microscopy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Atwell, W. J.: Differentiation and function of heterotopic autoplastic transplants of the amphibian hypophysis. Proc. Soc. exp. Biol. (N.Y.)33, 224–226 (1935)Google Scholar
  2. Atwell, W. J.: Functional transplants of the primordium of the epithelial hypophysis in Amphibia. Anat. Rec.68, 431–447 (1937)CrossRefGoogle Scholar
  3. Costoff, A., McShan, W. H.: Isolation and biological properties of secretory granules from rat anterior pituitary gland. J. Cell. Biol.43, 564–574 (1969)CrossRefPubMedGoogle Scholar
  4. Daikoku, S., Kinutani, M., Watanabe, Y. G.: Role of hypothalamus on development of adenohypophysis: An electron microscopic study. Neuroendocrinology11, 284–305 (1973)PubMedGoogle Scholar
  5. Driscoll, W. T., Eakin, R. M.: The effects of sucrose on amphibian development with special reference to the pituitary body. J. exp. Zool.129, 149–176 (1955)CrossRefGoogle Scholar
  6. Etkin, W.: Embryonic determination of the adenohypophysis in the wood frog,R. sylvatica. Anat. Rec.131, 548 (1958a)Google Scholar
  7. Etkin, W.: Independent differentiation in components of the pituitary complex in the wood frog. Proc. Soc. exp. Biol. (N.Y.)97, 388–393 (1958b)Google Scholar
  8. Ferrand, R.: Différenciation en greffe chorio-allantoïdienne de l'épithelium de la poche de Rathke d'embryons de caille et de poulet. C. R. Soc. Biol. (Paris)163, 2669–2672 (1969)Google Scholar
  9. Ferrand, R.: Étude expérimentale des facteurs de la différenciation cytologique de l'adénohypophyse chez l'embryon de poulet. Arch. Biol. (Liège)83, 297–371 (1972)Google Scholar
  10. Ferrand, R., Le Douarin, N.: Différenciation de tissu adénohypophysaire à partir de la poche de Rathke prélevée après le stade de la détermination chez l'embryon de Poulet et mise au contact de divers mésenchymes. C. R. Soc. Biol. (Paris)162, 2215–2218 (1968)Google Scholar
  11. Fink, G., Smith, G. C.: Ultrastructural features of the developing hypothalamo-hypophysial axis in the rat. A correlative study. Z. Zellforsch.119, 208–226 (1971)CrossRefPubMedGoogle Scholar
  12. Halász, B., Pupp, L., Uhlarik, S.: Hypophysiotrophic area in the hypothalamus. J. Endocr.25, 147–154 (1962)PubMedGoogle Scholar
  13. Hanaoka, Y.: The effects of posterior hypothalectomy upon the growth and metamorphosis of the tadpole ofRana pipiens. Gen. comp. Endocr.8, 417–431 (1967)CrossRefPubMedGoogle Scholar
  14. Hatakeyama, S.: Electron microscopic study of the anencephalic adenohypophysis with reference to the adrenocorticotrophs and their correlation with the functional differentiation of the hypothalamus during the foetal life. Endocr. jap.16, 187–203 (1969)Google Scholar
  15. Hyypä, M.: Differentiation of the hypothalamic nuclei during ontogenetic development in the rat. Z. Anat. Entwickl.-Gesch.129, 41–52 (1969)Google Scholar
  16. Ishikawa, H.: Differentiation of the anterior pituitary cells by neurohormones (in Japanese). Zool. Mag. (Tokyo)79, 405–406 (1970)Google Scholar
  17. Le Douarin, G., Ferrand, R.: Différenciation fonctionnelle de l'ébauche épithéliale de l'adénohypophyse isolée du plancher encéphalique: activité thyréotrope. C. R. Acad. Sci. (Paris)266, 697–699 (1968)Google Scholar
  18. Le Douarin, N., Ferrand, R., Le Douarin, G.: La différenciation de l'ébauche épithéliale de l'hypophyse séparée du plancher encéphalique et placée dans des mésenchymes hétérologues. C. R. Acad. Sci. (Paris)264, 3027–3029 (1967)Google Scholar
  19. Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol.39, 111–114 (1964)PubMedGoogle Scholar
  20. Moscona, H., Moscona, A.: The development in vitro of the anterior lobe of the embryonic chick pituitary. J. Anat. (Lond.)86, 278–286 (1952)Google Scholar
  21. Nikitovitch-Winer, M., Everett, J. W.: Functional restitution of pituitary grafts re-transplanted from kidney to median eminence. Endocrinology63, 916–930 (1958)PubMedGoogle Scholar
  22. Nikitovitch-Winer, M., Everett, J. W.: Histocytologic changes in grafts of rat pituitary on the kidney and upon re-transplantation under the diencephalon. Endocrinology65, 357–368 (1959)PubMedGoogle Scholar
  23. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell. Biol.17, 208–212 (1963)CrossRefPubMedGoogle Scholar
  24. Salazar, H., MacAulay, M. A., Charles, D., Pardo, M.: The human hypophysis in anencephaly. I. Ultrastructure of the pars distalis. Arch. Path.87, 201–211 (1969)PubMedGoogle Scholar
  25. Sobel, H.: The behaviour in vitro of dissociated embryonic pituitary tissue. J. Embryol. exp. Morph.6, 518–526 (1958)PubMedGoogle Scholar
  26. Trowell, O. A.: The culture of mature organs in a synthetic medium. Exp. Cell. Res.16, 118–147 (1959)CrossRefPubMedGoogle Scholar
  27. Yoshimura, F., Harumiya, K., Kiyama, H.: Light and electron microsopic studies of the cytogenesis of anterior pituitary cells in perinatal rats in reference to the development of target organs. Arch. histol. jap.31, 333–369 (1970)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Yûichi G. Watanabe
    • 1
  • Hideo Matsumura
    • 1
  • Shigeo Daikoku
    • 1
  1. 1.Department of Anatomy, School of MedicineTokushima UniversityKuramoto, TokushimaJapan

Personalised recommendations