A rapid method to differentiate between five species of the genusSaccharomyces
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Long-chain fatty acids of 8 yeast strains representing 5 species of the genusSaccharomyces, were extracted from yeast cells by saponification and analyzed as methyl esters by gasliquid chromatography (GLC). When these species were grown under standard conditions, each produced a distinctive mean fatty-acid “fingerprint” characterized by certain fatty acid compositions. With this method, it was possible to distinguish between the 5 species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguishing between species ofSaccharomyces.
KeywordsMethyl Ester Chromatography Gasliquid Chromatography Methyl Ester
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