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Applied Microbiology and Biotechnology

, Volume 23, Issue 6, pp 499–501 | Cite as

A rapid method to differentiate between five species of the genusSaccharomyces

  • Johan L. F. Kock
  • Michele Cottrell
  • Pieter M. Lategan
Short contribution

Summary

Long-chain fatty acids of 8 yeast strains representing 5 species of the genusSaccharomyces, were extracted from yeast cells by saponification and analyzed as methyl esters by gasliquid chromatography (GLC). When these species were grown under standard conditions, each produced a distinctive mean fatty-acid “fingerprint” characterized by certain fatty acid compositions. With this method, it was possible to distinguish between the 5 species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguishing between species ofSaccharomyces.

Keywords

Methyl Ester Chromatography Gasliquid Chromatography Methyl Ester 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Johan L. F. Kock
    • 1
  • Michele Cottrell
    • 1
  • Pieter M. Lategan
    • 1
  1. 1.Department of MicrobiologyUniversity of the Orange Free StateRepublic of South Africa

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