A rapid method to differentiate between five species of the genusSaccharomyces
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Long-chain fatty acids of 8 yeast strains representing 5 species of the genusSaccharomyces, were extracted from yeast cells by saponification and analyzed as methyl esters by gasliquid chromatography (GLC). When these species were grown under standard conditions, each produced a distinctive mean fatty-acid “fingerprint” characterized by certain fatty acid compositions. With this method, it was possible to distinguish between the 5 species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguishing between species ofSaccharomyces.
KeywordsMethyl Ester Chromatography Gasliquid Chromatography Methyl Ester
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- Abel K, Schmertzing H, Peterson JI (1963) Classification of micro-organisms by analysis of chemical composition. I. Feasibility of utilizing gas chromatography. J Bacteriol 85:1039–1044Google Scholar
- Barnett JA, Payne RW, Yarrow D (1983) Yeasts: Characteristics and identification. Cambridge University Press, New YorkGoogle Scholar
- Dees SB, Moss CW (1975) Cellular fatty acids ofAlcaligenes andPseudomonas species isolated from clinical specimens. J Clin Microbiol 1:414–419Google Scholar
- Gunasekaran M, Hughes WT (1980) Gas-liquid chromatography: A rapid method for identification of different species ofCandida. Mycologia 72:505–511Google Scholar
- Lennette EH, Spaulding EH, Truant JP (1974) Manual of Clinical Microbiology. American Society for Microbiology. Washington, D.C.Google Scholar
- Moss CW, Dees SB (1976) Cellular fatty acids and metabolic products ofPseudomonas species obtained from clinical specimens. J Clin Microbiol 4:492–502Google Scholar
- Moss CW, Kaltenbach CM (1974) Production of glutaric acid: A useful criterion for differentiatingPseudomonas diminuta fromPseudomonas vesiculare. Appl Microbiol 27:437–439Google Scholar
- Rattray JBM, Schibeci A, Kidby DK (1975) Lipids of yeasts. Bacteriol Rev 39:197–231Google Scholar
- Shaw N (1974) Advances in Applied Microbiology. Academic Press, New York/London, pp 63–108Google Scholar
- Thoen CO, Karlson AG, Ellefson RD (1972) Differentiation betweenMycobacterium kanasii andMycobacterium marinum by gas liquid chromatographic analysis of cellular fatty acids. Appl Microbiol 24:1009–1010Google Scholar