Applied Microbiology and Biotechnology

, Volume 23, Issue 6, pp 445–448 | Cite as

Comparison of different methods for protein determination inAspergillus niger mycelium

  • Katarina Jernejc
  • Aleksa Cimerma
  • Anton Perdih


Protein contents were determined in submerged as well as in surface-grown citric acid producingAspergillus niger mycelia. Various methods (Kjeldahl, Biuret, Lowry and Coomassie Blue) for protein determination were compared. The Biuret method seemed to be more suitable than the others for true protein determination in mycelia. The Lowry method gave lower results in all cases. The Coomassie Blue method did not prove suitable for the material used.


Protein Content Citric Acid Coomassie Protein Determination Lowry Method 
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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Katarina Jernejc
    • 1
  • Aleksa Cimerma
    • 1
  • Anton Perdih
    • 2
  1. 1.Boris Kidrič Institute of ChemistryLjubljanaYugoslavia
  2. 2.Department of Chemistry and Chemical TechnologyEdvard Kardelj University of LjubljanaLjubljanaYugoslavia

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