Comparison of different methods for protein determination inAspergillus niger mycelium
- 221 Downloads
Protein contents were determined in submerged as well as in surface-grown citric acid producingAspergillus niger mycelia. Various methods (Kjeldahl, Biuret, Lowry and Coomassie Blue) for protein determination were compared. The Biuret method seemed to be more suitable than the others for true protein determination in mycelia. The Lowry method gave lower results in all cases. The Coomassie Blue method did not prove suitable for the material used.
KeywordsProtein Content Citric Acid Coomassie Protein Determination Lowry Method
Unable to display preview. Download preview PDF.
- Burton K (1957) Protein and nucleic acid synthesis in bacteriophage multiplication. Biochem J 62:315–323Google Scholar
- Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766Google Scholar
- Kuno H, Kihara KH (1967) Microassay of protein with membrane filter. Nature (London) 215:974–975Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement. J Biol Chem 193:265–275Google Scholar