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Inhibition of TNF-α induced cell death in human umbilical vein endothelial cells and Jurkat cells by protocatechuic acid

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Abstract

The Chinese herb radix Salviae miltiorrhizae (RSM) is used in traditional Chinese medicine as a treatment for cardiovascular and cerebrovascular diseases. Several components of the plant extract from salvia mitorrhiza bunge have been determined previously, one of which is protocatechuic acid (PAC). It has been found, in the study, that PAC inhibited TNF-α-induced cell death of human umbilical vein endothelial cells (HUVECs) and Jurkat cells in a concentration of 100μM when applied 2 h prior to TNF-α exposure. Molecular studies revealed that PAC activated NF-κB with a maximum effect after 30 min of treatment. Inhibition of NF-κB action by MG132 and NF-κB inhibitory peptide suppressed the cell-protective effect of PAC. Further, degradation of lkBα occurred in response to PAC treatment. The results provide evidence that activation of NF-κB plays an important role in mediating the cell-protecting effect of PAC on HUVECs and Jurkat cells. Further studies are required to test whether PAC, a component of radix salviae miltiorrhizae, could be useful in preventing in vivo cell death resulting from cardiovascular or cerebrovascular diseases.

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Authors and Affiliations

  1. Institute of Pathology, University of RWTH, Aachen, Germany

    J. Zhou-Stache, R. Buettner, C. Mittermayer &  A. K. Bosserhoff

  2. Department of Cell Biophysics, FH Aachen Abteilung Jülich, Germany

    J. Zhou-Stache & G. Artmann

  3. Institute of Pathology, University of Regensburg, Germany

    A. K. Bosserhoff

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  1. J. Zhou-Stache
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  2. R. Buettner
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  3. G. Artmann
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  4. C. Mittermayer
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  5. A. K. Bosserhoff
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Correspondence to A. K. Bosserhoff.

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Zhou-Stache, J., Buettner, R., Artmann, G. et al. Inhibition of TNF-α induced cell death in human umbilical vein endothelial cells and Jurkat cells by protocatechuic acid. Med. Biol. Eng. Comput. 40, 698–703 (2002). https://doi.org/10.1007/BF02345308

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  • DOI: https://doi.org/10.1007/BF02345308

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