Medical and Biological Engineering and Computing

, Volume 38, Issue 6, pp 686–689 | Cite as

Preparation of a pure autologous biodegradable fibrin matrix for tissue engineering

  • A. Haisch
  • A. Loch
  • J. David
  • A. Pruß
  • R. Hansen
  • M. Sittinger
Cellular Engineering

Abstract

Parallel to the growing role of tissue engineering, the need for cell embedding materials, which allow cells to stabilise in a three-dimensional distribution, has increased. Although several substances have been tested, fibrin is thus far the only one that permits the clinical application of cultured tissue. To date, can cause severe immunological side effects. The objective of this study was to explore the practicability of obtaining autologous thrombin from a single patient in an adequate concentration and amount. Fibrinogen was cryoprecipitated from 200 ml of freshly-frozen plasma. Thrombin was isolated from the supernatant through ionexchange chromatography. The thrombin was first bound to Sephadex A-50 and then eluated using 2ml of a salt buffer (2.0M NaCl in 0.015M trisodiumcitrate, pH 7.0). The activity of the thrombin (51 NIH ml−1 to 414 NIH ml−1) reached levels comparable to those in commercially available fibrin glues (4–500 NIH ml−1). The study has shown that it is possible to obtain a sufficient amount of autologous thrombin from a single donor to create a fibrin matrix of high efficiency without the risk of immunological and infectious side effects.

Keywords

Tissue engineering Autologous fibrinogen Thrombin Matrix 

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Copyright information

© IFMBE 2000

Authors and Affiliations

  • A. Haisch
    • 1
    • 2
  • A. Loch
    • 1
    • 2
  • J. David
    • 2
    • 3
  • A. Pruß
    • 4
  • R. Hansen
    • 5
  • M. Sittinger
    • 2
    • 6
    • 7
  1. 1.Department of Otorhinolaryngology, Head and Neck SurgeryCharité, Medical Faculty of the Humboldt UniversityBerlin
  2. 2.Tissue Engineering LaboratoryCharité, Medical Faculty of the Humboldt UniversityBerlin
  3. 3.Biotechnological Faculty of the Technical University of BerlinBerlin
  4. 4.Institute of Transfusion MedicineCharté, Medical Faculty of the Humboldt UniversityBerlin
  5. 5.Institute of Laboratory Medicine and PathobiochemistryCharité, Medical Faculty of the Humboldt UniversityBerlin
  6. 6.Department of RheumatologyCharité, Medical Faculty of the Humboldt UniversityBerlin
  7. 7.German Rheumatism Research CenterBerlin

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