Abstract
From the blue seed coats ofOphiopogon jaburan, a new flavonol glycoside was isolated as needles and determined to be kaempferol 3-O-β-d-galactoside-4′-O-β-d-glucoside (OK-2) by UV and NMR spectral analyses. OK-2 and kaempfrol 3, 4′-di-O-β-d-glucoside (OK-1), which was detected previously, in the blue seed coat were present in a molar ratio of about 13:7. OK-2 was newly found as a factor causing the blueing effects on ophionin which is a main anthocyanin in the blue seed coats. The mixture of 4.8×10−3 M OK-2 and 2.5×10−3 M ophionin in Mcllvaine's buffer solution (pH 5.6) showed stable blue color, and the absorption spectrum of the mixture showed two absorption peaks and a shoulder in visible reasion, coinciding with that of the fresh blue seed coat. The effect of ophionin and OK-2 co-pigmentation on the blue color of seed coat ofO. jaburan was discussed.
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Abbreviations
- OK-1:
-
kaempferol 3, 4′-di-O-β-d-glucoside
- OK-2:
-
kaempferol 3-O-β-d-galactoside-4′-O-β-d-glucoside
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Yoshitama, K., Kawasoe, T. & Ishikura, N. Isolation of a new flavonol glycoside and its effects on the blue color of seed coats ofOphiopogon jaburan . J. Plant Res. 106, 223–227 (1993). https://doi.org/10.1007/BF02344589
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DOI: https://doi.org/10.1007/BF02344589