Journal of Plant Research

, Volume 109, Issue 4, pp 403–408 | Cite as

Spatial distribution of cpDNA and mtDNA haplotypes in a hybrid zone betweenPinus pumila andP. parviflora var.pentaphylla (Pinaceae)

  • Yasuyuki Watano
  • Michio Imazu
  • Tatemi Shimizu
Original Articles


Pinus species exhibit paternal chloroplast inheritance and maternal mitochondrial inheritance. This independent inheritance of two cytoplasmic genomes provides an exceptional environment for discriminating female (seeds) and male (pollen) components of gene flow across hybridizing species. We obtained mitochondrial genetic markers diagnostic toP. parviflora var.pentaphylla andP. pumila by PCR amplification of the intron ofnad1 on mtDNA, and examined the spatial-distribution pattern of the mtDNA haplotypes in a hybrid zone betweenP. parviflora var.pentaphylla andP. pumila in the Tanigawa Mountains of Japan. These data, in conjunction with previous information on cpDNA haplotypes and needle morphology, revealed contrastive patterns of introgression of two cytoplasmic genomes. CpDNA introgression has occurred uni-directionally fromP. parviflora var.pentaphylla toP. pumila. Conversely, mtDNA introgression has occurred in the opposite direction, fromP. pumila toP. parviflora var.pentaphylla. Levels of introgression are roughly equivalent for cpDNA and mtDNA. The contrastive spatial distribution pattern of cpDNA and mtDNA haplotypes could be caused by differential movement of seeds and pollen for interspecific genetic exchange.

Key words

Choroplast DNA Introgressive hybridization Mitochondrial DNA Pinus 


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Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Yasuyuki Watano
    • 1
  • Michio Imazu
    • 2
  • Tatemi Shimizu
    • 1
  1. 1.Department of Biology, Faculty of ScienceKanazawa UniversityKanazawaJapan
  2. 2.Institute of Agriculture and ForestryUniversity of TsukubaTsukubaJapan

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