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Journal of Plant Research

, Volume 108, Issue 3, pp 395–397 | Cite as

Diamine oxidase from millet catalyzes the oxidation of 1, 3-diaminopropane

  • Howlader M. A. Awal
  • Eiji Hirasawa
Short Communication

Abstract

Diamine oxidase was purified sixty-fold from millet shoots. The partially purified enzyme of 150 kDa oxidized 1, 3-diaminopropane (1, 3-DAP) to 3-aminopropionaldehyde. The Km values were 9.1×10−5M for 1, 3-DAP and 6.3×10−4M for putrescine. Extracts of shoots of prosomillet, maize and barley also contained an activity that oxidized 1, 3-DAP.

Key words

3-Aminopropionaldehyde Diamine oxidase 1, 3-Diaminopropane Setaria italica 

Abbreviations

APA

aminopropionaldehyde

Da

dalton

DAO

diamine oxidase

DAP

diaminopropane

PAO

polyamine oxidase

Pi

phosphate

PAGE

polyacrylamide gel electrophoresis

PUT

putrescine

SPD

spermidine

SPM

spermine

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Copyright information

© The Botanical Society of Japan 1995

Authors and Affiliations

  • Howlader M. A. Awal
    • 1
  • Eiji Hirasawa
    • 1
  1. 1.Department of Biology, Faculty of ScienceOsaka City UniversityOsakaJapan

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