Journal of Plant Research

, Volume 108, Issue 2, pp 209–216 | Cite as

Chromatin arrangements in intact interphase nuclei examined by laser confocal microscopy

  • Junko Noguchi
  • Kiichi Fukui
Original Articles

Abstract

Using a laser confocal microscope, chromatin arrangements in intact interphase nuclei were investigated in four plant species. Chromosomes in these plants have specific segments that can be stained with the fluorescent dye chromomycin A3 (CMA). We stained centromeres inHordeum vulgare, sub-telomeric regions inSecale cereale, satellites inChrysanthemum multicore, and the satellites and the short arms of chromosomes with satellites inHemerocallis middendorfii. The following points were shown: (1) In mitotic interphase nuclei, the centromere and the telomeres of both arms touched the nuclear membrane and had evident polarity. Some CMA-bodies in sub-telomeric regions do not contact the nuclear membrane. (2) Differentiated nuclei had a non-random construction. Polarity of chromosomes is maintained, however, the chromosomes are far apart from the nuclear membrane. (3) Associations in sub-telomeric regions in the interphase nuclei ofSecale cereale were probably due to the association of heterochromatic regions with identical repeated sequences rather than telomere associlations. (4) In interphase nuclei ofChrysanthemum multicore, satellites fused during interphase.

Key words

Centromere Chromatin arrangement Laser confocal microscopy Rabl orientation Satellite association Sub-telomeric association 

Abbreviation

CMA

chromomycin A3

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Copyright information

© The Botanical Society of Japan 1995

Authors and Affiliations

  • Junko Noguchi
    • 1
  • Kiichi Fukui
    • 2
  1. 1.Department of Botany, Faculty of ScienceKyoto UniversityKyotoJapan
  2. 2.Department of BreedingHokuriku National Agricultural, Experimental StationJoetsuJapan

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