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Bsr-REMI: An improved method for gene tagging using a new vector inDictyostelium

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Abstract

Using a plasmid pBsr2 which carries a blasticidin S-resistant gene, we have improved the method of REMI (restriction enzyme-mediated integration) provided for insertional mutagenesis inDictyostelium discoideum (bsr-REMI). To confirm usefulness of thebsr-REMI, transformation efficiency, copy number of integrated DNA, and randomness of integration into genome were examined.

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Authors and Affiliations

  1. Institute of Biological Sciences, University of Tsukuba, 305, Tsukuba, Ibaraki, Japan

    Takahiro Morio, Kaichiro Yanagisawa & Yoshimasa Tanaka

  2. Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, 153, Tokyo, Japan

    Hiroyuki Adachi & Kazuo Sutoh

Authors
  1. Takahiro Morio
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  2. Hiroyuki Adachi
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  3. Kazuo Sutoh
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  4. Kaichiro Yanagisawa
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  5. Yoshimasa Tanaka
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Morio, T., Adachi, H., Sutoh, K. et al. Bsr-REMI: An improved method for gene tagging using a new vector inDictyostelium . J. Plant Res. 108, 111–114 (1995). https://doi.org/10.1007/BF02344314

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  • DOI: https://doi.org/10.1007/BF02344314

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