Journal of Plant Research

, Volume 109, Issue 1, pp 119–125 | Cite as

Proton pumps of the vacuolar membrane in growing plant cells

  • Masayoshi Maeshima
  • Yoichi Nakanishi
  • Chie Matsuura-Endo
  • Yoshiyuki Tanaka
JPR Symposium


Plant growth results from the division, enlargement and specialization of cells. The two processes of the enlargement and the differentiation of cells are not spatially separated in plant tissue. We focus our attention here on the enlargement and elongation of cells. In most cases, growing plant cells contain a large central vacuole. The acid growth theory is based on the space-filling function of the large vacuole. The active transport systems in the vacuolar membrane are essential for maintenance of high osmotic pressure and for the expansion of the vacuole. The secondary active transport systems of the vacuole for sugars and ions are driven by the proton-motive force which is generated by the vacuolar H+-ATPase and H+-translocating inorganic pyrophosphatase. In this review, the relationship between cell elongation and these enzymes of the vacuolar membrane is emphasized.

Key words

Cell elongation H+-ATPase H+-Pyrophosphatase Proton pump Vacuole Vigna radiata Water channel 





proton-translocating inorganic pyrophosphatase


inorganic pyrophosphate


tonoplast intrinsic protein


an integral membrane protein of the radish vacuole with a molecular mass of 23 kD.


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Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Masayoshi Maeshima
    • 1
  • Yoichi Nakanishi
    • 1
  • Chie Matsuura-Endo
    • 2
  • Yoshiyuki Tanaka
    • 3
  1. 1.Laboratory of Biochemistry, School of Agricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Center of Crop ScienceHokkaido National Agricultural Experiment StationHokkaidoJapan
  3. 3.Chemical Tolerance LaboratoryNational Institute of Agrobiological ResourcesIbarakiJapan

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