Journal of Plant Research

, Volume 109, Issue 1, pp 61–65 | Cite as

Changes in the relationship between actin filaments and the plasma membrane in culturedZinnia cells during tracheary element differentiation investigated by using plasma membrane ghosts

  • Hitoshi Kobayashi
Short Communication


Aggregates of actin filaments appear immediately before secondary wall thickening during tracheary element differentiation in isolatedZinnia cells. An analysis of plasma membrane ghosts revealed that the aggregates were bound to the plasma membrane. The properties of the binding of actin filaments to the plasma membrane were investigated in this system.

Key words

Actin filament Cytodifferentiation Plasma membrane ghost Tracheary element Zinnia elegans 


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  1. Akashi, T. andShibaoka, H. 1991. Involvement of transmembrane proteins in association of cortical microtubules with the plasma membrane in tobacco BY-2 cells. J. Cell Sci.98: 169–174.Google Scholar
  2. Fukuda, H. andKobayashi, H. 1989. Dynamic organization of the cytoskeleton during tracheary-element differentiation. Develop. Growth Differ.31: 9–16.CrossRefGoogle Scholar
  3. Fukuda, H. andKomamine, A. 1980. Establishment of an experimental system for the study of tracheary element differentiation from single cells isolated from the mesophyll ofZinnia elegans. Plant Physiol.65: 57–60.Google Scholar
  4. Fukuda, H. andKomamine, A. 1985. Cytodifferentiation.In I.K. Vasil, ed., Cell growth, Nutrition, Cytodifferentiation, and Cryopreservation. vol. 2, Academic Press, New York, pp. 149–212.Google Scholar
  5. Giddings, T.H. andStaehelin, L.A. 1988. Spatial relationship between microtubules and plasma-membrane rosettes during the deposition of primary wall microfibrils inClosterium sp. Planta173: 22–30.CrossRefGoogle Scholar
  6. Hardham, A.R. andGunning, B.E.S. 1978. Structure of cortical microtubule arrays in plant cells. J. Cell Biol.77: 14–34.CrossRefPubMedGoogle Scholar
  7. Kakimoto, T. andShibaoka, H. 1986. Calcium-sensitivity of cortical microtubules in the green algaMougeotia. Plant Cell Physiol.27: 91–101.Google Scholar
  8. Kakimoto, T. andShibaoka, H. 1987a. Actin filaments and microtubules in the preprophase band and phragmoplast of tobacco cells. Protoplasma140: 151–156.CrossRefGoogle Scholar
  9. Kakimoto, T. andShibaoka, H. 1987b. A new method for preservation of actin filaments in higher plant cells. Plant Cell Physiol.28: 1581–1585.Google Scholar
  10. Katsuta, J. andShibaoka, H. 1992. Inhibition by kinase inhibitors of the development and the disappearance of the preprophase band of microtubules in tobacco BY-2 cells. J. Cell Sci.103: 397–405.Google Scholar
  11. Kobayashi, H., Fukuda, H. andShibaoka, H. 1987. Reorganization of actin filaments associated with the differentiation of tracheary elements inZinnia mesophyll cells. Protoplasma138: 69–71.CrossRefGoogle Scholar
  12. Kobayashi, H., Fukuda, H. andShibaoka, H. 1988. Interrelation between the spatial disposition of actin filaments and microtubules during the differentiation of tracheary elements in culturedZinnia cells. Protoplasma143: 29–37.CrossRefGoogle Scholar
  13. Lancelle, S.A., Callaham, D.A. andHepler, P.K. 1986. A method for rapid freeze fixation of plant cells. Protoplasma131: 153–165.CrossRefGoogle Scholar
  14. Lloyd, C.W., Slabas, A.R., Powell, A.J. andLowe, S.B. 1980. Microtubules, protoplasts and plant cell shape. An immunofluorescent study. Planta147: 500–505.CrossRefGoogle Scholar
  15. Marchant, H.J. andHines, E.R. 1979. The role of microtubules and cell-wall deposition in elongation of regenerating protoplasts ofMougeotia. Planta146: 41–48.CrossRefGoogle Scholar
  16. Parthasarathy, M.V., Perdue, T.D., Witztum, A. andAlvernaz, J. 1985. Actin network as a normal component of the cytoskeleton in many vascular plant cells. Am. J. Bot.72: 1318–1323.Google Scholar
  17. Schliwa, M. 1986. The Cytoskeleton. An Introductory Survey. Springer-Verlag, Wien New York.Google Scholar
  18. Seagull, R.W. andHeath, I.B. 1980. The organization of cortical microtubule arrays in the radish root hair. Protoplasma103: 205–229.Google Scholar
  19. Sonobe, S. 1990. ATP-dependent depolymerization of cortical microtubules by an extract in tobacco BY-2 cells. Plant Cell Physiol.31: 1147–1153.Google Scholar
  20. Sonobe, S. andShibaoka, H. 1989. Cortical fine actin filaments in higher plant cells visualized by rhodaminephalloidin after pretreatment with m-maleimidobenzoyl N-hydroxysuccinimide ester. Protoplasma148: 80–86.CrossRefGoogle Scholar
  21. Sonobe, S. andTakahashi, T. 1994. Association of microtubules with the plasma membrane of tobacco BY-2 cells in vitro. Plant Cell Physiol.35: 451–460.Google Scholar
  22. Spudich, J.A. andWatt, S. 1971. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the tropomyosin-troponin complex with actin and proteolytic fragments of myosin. J. Biol. Chem.246: 4866–4871.PubMedGoogle Scholar
  23. Staiger, C.J. andSchliwa, M. 1987. Actin localization and function in higher plants. Protoplasma141: 1–12.CrossRefGoogle Scholar
  24. Staiger, C.J. andCande, W.Z. 1991. Microfilament distribution in maize meiotic mutants correlates with microtubule organization. Plant Cell3: 637–644.CrossRefPubMedGoogle Scholar
  25. Wang, Y. andYan, L. 1991. The membrane proteins of leaf cell membrane ofVicia faba. Chinese Sci. Bulletin36: 862–866.Google Scholar

Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Hitoshi Kobayashi
    • 1
  1. 1.Department of Biology, Faculty of ScienceOsaka UniversityToyonaka, OsakaJapan

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