Journal of Plant Research

, Volume 109, Issue 1, pp 29–36 | Cite as

Electrophoretic and cytological evidence for genetic heterogeneity and the hybrid origin ofAthyrium oblitescens

  • Tomoaki Kurihara
  • Yasuyuki Watano
  • Masayuki Takamiya
  • Tatemi Shimizu
Original Articles


Athyrium oblitescens, a putative hybrid species, was examined electrophoretically and cytologically to clarify its origin. Allozyme data showed thatA. oblitescens consisted of at least three allozyme types, designated Type C, Type O and Type W. Genotypic compositions of Type C and Type W suggested that they were derived from independent hybridizations betweenA. otophorum andA. clivicola and betweenA. otophorum andA. wardii, respectively. Contrary to previous reports of tetraploidy inA. oblitescens and its hypothesized parent species, cytological observation revealed that Type C and Type W were both hexaploids. Possible pathways for the origin of these hexaploids are proposed. Type O was not genetically distinct fromA. otophorum by either electrophoretic or cytological analyses, and thus there was no evidence of hybrid origin.

Key words

Allozyme Athyrium oblitescens Chromosome number Hexaploidy Reticulate evolution 


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Copyright information

© The Botanical Society of Japan 1996

Authors and Affiliations

  • Tomoaki Kurihara
    • 1
  • Yasuyuki Watano
    • 1
  • Masayuki Takamiya
    • 2
  • Tatemi Shimizu
    • 1
  1. 1.Department of Biology, Faculty of ScienceKanazawa UniversityKanazawaJapan
  2. 2.Department of Biological Science, Faculty of ScienceKumamoto UniversityKumamotoJapan

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