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Journal of Plant Research

, Volume 107, Issue 4, pp 365–369 | Cite as

In situ hybridization to metaphase chromosomes in six species ofPhaseolus andVigna using ribosomal DNA as the probe

  • Jianyu Zheng
  • Kohei Irifune
  • Kouji Hirai
  • Masashi Nakata
  • Ryuso Tanaka
  • Hiromichi Morikawa
Original Articles

Abstract

In situ hybridization with a biotin-labeled rice ribosomal DNA (rDNA) probe to the somatic metaphase chromosomes of six species ofPhaseolus andVigna (P. angularis, P. calcaratus, P. coccineus, P. vulgaris, V. sesquipedalis andV. sinensis) was done to determine the sites of rDNA. Hybridization signals were present in the terminal and subterminal chromosome regions of each of the six species. The number of rDNA sites was two inP. angularis andP. calcaratus, four inP. coccineus andP. vulgaris, and six inV. sesquipedalis andV. sinensis.

Key words

In situ hybridization Phaseolus Ribosomal DNA Somatic metaphase chromosome Vigna 

Abbreviations

CMA

chromomycin A3

DAPI

4′-6-diamidino-2-phenylindole

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Copyright information

© The Botanical Society of Japan 1994

Authors and Affiliations

  • Jianyu Zheng
    • 1
  • Kohei Irifune
    • 1
  • Kouji Hirai
    • 2
  • Masashi Nakata
    • 3
  • Ryuso Tanaka
    • 1
  • Hiromichi Morikawa
    • 1
  1. 1.Faculty of ScienceHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Shobara Kakuchi High SchoolShobara, HiroshimaJapan
  3. 3.Botanic Gardens of ToyamaToyamaJapan

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