Summary
The purpose of this study was to examine plasma and intraerythrocyte lactate concentrations during graded exercise in humans. Seven adult volunteers performed a maximum O2 uptake (\(\dot V_{O_{_2 max} } \)) test on a cycle ergometer. Plasma and intraerythrocyte lactate concentrations (mmol · L−1 of plasma or cell water) were determined at rest, during exercise, and at 15-min post-exercise. The results show that plasma and intraerythrocyte lactate concentrations were not significantly different from each other at rest or moderate (⩽50%\(\dot V_{O_{_2 max} } \)) exercise. However, the plasma concentrations were significantly increased over the intraerythrocyte levels at 75% and 100%\(\dot V_{O_{_2 max} } \). The plasma to red cell lactate gradient reached a mean (±SE) 1.7±0.4 mmol · L−1 of H2O at exhaustion, and was linearly (r=0.84) related to the plasma lactate concentration during exercise. Interestingly, at 15-min post-exercise the direction of the lactate gradient was reversed, with the mean intraerythrocyte concentration now being significantly increased over that found in the plasma. These results suggest that the erythrocyte membrane provides a barrier to the flux of lactate between plasma and red cells during rapidly changing blood lactate levels. Furthermore, these data add to the growing body of research that indicates that lactate is not evenly distributed in the various water compartments of the body during non-steady state exercise.
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References
Belcastro AN, Bonen A (1975) Lactic acid removal rates during controlled and uncontrolled recovery exercise. J Appl Physiol 39:932–936
Bromberg, PA, Theordore J, Robin ED, Jensen WN (1965) Anion and hydrogen ion distribution in human blood. J Lab Clin Med 66:464–475
Brooks GA, Gaesser GA (1980) End points of lactate and glucose metabolism after exhausting exercise. J Appl Physiol 49:1057–1069
Buono MJ, Roby FB (1982) Acid-base, metabolic, and ventilatory responses to repeated bouts of exercise. J Appl Physiol 53:436–439
Consolazio CF, Johnson RE, Pecora LJ (1963) Physiological measurements of metabolic functions in man. McGraw-Hill, Inc., New York, pp 125–126
Daniel SS, Morishima HO, James LS, Adamsons K Jr (1964) Lactate and pyruvate gradient between red blood cells and plasma during acute asphyxia. J Appl Physiol 19:1100–1104
Decker DG, Rosenbaum JD (1942) The distribution of lactic acid in human blood. Am J Physiol 138:7–11
Deuticke B (1982) Monocarboxylate transport in erythrocytes. J Membr Biol 70:89–103
Devadatta SC (1934) Distribution of lactate between the corpuscles and the plasma in blood. Quart J Exp Physiol 24:295–303
Dubinsky WP, Racker E (1978) The mechanism of lactate transport in human erythrocytes. J Membrane Biol 44:25–36
Fitzsimons EJ, Sendroy J Jr (1961) Distribution of electrolytes in human blood. J Biol Chem 236:1595–1601
Freund H, Zouloumian P, Enguelle SO, Lampert E (1984) Lactate kinetics after maximal exercise in man. Med Sport Sci 17:9–24
Halestrap AP (1976) Transport of pyruvate and lactate into human erythrocytes. Biochem J 156:193–207
Halestrap AP, Denton RM (1974) Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by a-cyano-4-hydroxycinnamate. Biochem J 138:313–316
Huckabee WE (1956) Control of concentration gradients of pyruvate and lactate across cell membranes in blood. J Appl Physiol 9:163–170
Johnson RE, Edwards HT, Dill DB, Wilson JW (1945) Blood as a physicochemical system: the distribution of lactate. J Biol Chem 157:461–473
Jorfeldt L, Juhlin-Dannfelt A, Karlson J (1978) Lactate release in relation to tissue lactate in human skeletal muscle during exercise. J Appl Physiol 44:350–352
Miller RG, Jr (1981) Simultaneous statistical inference, 2nd edition. Springer, Berlin Heidelberg New York pp 67–70
Ohira Y, Ito A, Ikawa S (1977) Concentration of water content and solute concentration in blood during hemoconcentration. J Appl Physiol 42:739–743
Piquard F, Shaefer A, Dellenbach P, Haberey P (1980) Rapid bedside estimation of plasma and whole blood lactic acid. Intens Care Med 7:35–38
Sahlin K, Alvestrand A, Brandt R, Hultman E (1978) Intracellular pH and bicarbonate concentration in human musle during recovery from exercise. J Appl Physiol 45:474–480
Wilmore JH, Costill DL (1974) Semi-automated systems approach to the assessment of oxygen uptake during exercise. J Appl Physiol 36:618–620
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Buono, M.J., Yeager, J.E. Intraerythrocyte and plasma lactate concentrations during exercise in humans. Europ. J. Appl. Physiol. 55, 326–329 (1986). https://doi.org/10.1007/BF02343807
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DOI: https://doi.org/10.1007/BF02343807