Abstract
Sets of isomeric anti-lactose antibodies with specificity for the lactose units of a cell wall polysaccharide fromStreptococcus faecalis strain N were induced in rabbits immunized with a vaccine of nonviable cells of the organism. Such sets of anti-lactose antibodies were isolated from the serum of immunized animals by affinity chromatography on lactosyl-Sepharose. Gel electrofocusing experiments showed that the preparations consisted of multiprotein components. One preparation of antibodies of 13 isomers was separated into homogeneous components by liquid isoelectrofocusing. The individual isomeric antibodies exhibit specificity for the lactose units of the antigenic polysaccharide, possess isoelectric points in the range of 5.9–8.0, and belong to the IgG class of immunoglobulins, and each member yields one light chain and one heavy chain on dissociation in sodium dodecyl sulfate (SDS) and mercaptoethanol. These results have been interpreted as evidence for the assembly of the chains of isomeric antibodies by a single-chain pairing mechanism.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AwdehZ. L., WilliamsonA. R., and AskonasB. A. (1969).Biochem. J. 116, 241–248.
BothwellA. L. M., PaskindM., RethM., Imanishi-KariT., RajewskyK., and BaltimoreD. (1981).Cell 24, 625–637.
CapraJ. D., TungA. S., and NisonoffA. (1977).J. Immunol. 119, 993–999.
Commission on Biochemical Nomenclature (1973). Elsevier Scientific Publishing Co., Amsterdam, The Netherlands.
CuatrecasasP. (1970).J. Biol. Chem. 245, 3059–3065.
DavisB. J. (1964).Ann. N.Y. Acad. Sci. 121, 404–427.
DoerrP., and ChrambachA. (1971).Anal. Biochem. 42, 96–107.
EdelmanG. M., OlinsD. E., GallyJ. A., and ZinderN. D. (1963).Proc. Natl. Acad. Sci. USA 50, 753–761.
EisenH. H., and ReillyE. B. (1985).Annu. Rev. Immunol. 3, 337–365.
GibsonD. M. (1984).Mol. Immunol. 21, 421–432.
HoffmanD. R., GrossbergA. L., and PressmanD. (1972).J. Immunol. 108, 18–25.
HoodL. (1982). inMolecular Genetic Neuroscience (SchmittF. O., BirdS. J., and BloomF. E., eds.), Raven Press, New York, pp. 75–85.
LammM. E. (1976).Adv. Immunol. 22, 223–290.
MartinR. G., and AmesB. N. (1961).J. Biol. Chem. 236, 1372–1379.
MitchellK. F., KarushF., and MorganD. O. (1977).Immunochemistry 14, 161–164.
PazurJ. H. (1982).Carbohyd. Res. 107, 243–254.
PazurJ. H., AndersonJ. S. and KarakawaW. W. (1971).J. Biol. Chem. 246, 1793–1798.
PazurJ. H., CepureA., KaneJ. A., and HellerquistC. G. (1973).J. Biol. Chem. 248, 279–284.
PazurJ. H., DreherK. L., and ForsbergL. S. (1978). J. Biol. Chem.253, 1832–1837.
PazurJ. H., DreherK. L., TayM. E., and PazurB. A. (1983).Biochem. Biophys. Res. Commun. 113, 555–561.
PazurJ. H., and ForsbergL. S. (1980).Methods Carbohyd. Chem. 8, 107–116.
PazurJ. H., KleppeK., and AndersonJ. S. (1962).Biochim. Biophys. Acta 65, 369–372.
PazurJ. H., MillerK. B., DreherK. L., and ForsbergL. S. (1976).Biochem. Biophys. Res. Commun. 70, 545–550.
PazurJ. H., TominagaY., and KellyS. (1984).J. Protein Chem. 3, 49–62.
PerlmutterR. M., BrilesD. E., GreveJ. M., and DavieJ. M. (1978).J. Immunol. 121, 149–158.
PerlmutterR. M., CrewsS. T., DouglasR., SorensenG., JohnsonN., NiveraNadine, GearhartP. J., and HoodL. (1984).Adv. Immunol. 35, 1–37.
TonegawaS. (1983).Nature (Lond.) 302, 575–581.
UhrJ. W. (1970).Cell Immunol. 1, 228–244.
ValmetE. (1968).Science Tools 15, 8–11.
WaterfieldM. D., PrahlJ. W., HoodL. E., KindtT. J., and KrauseR. M. (1972).Nature New Biol. 240, 215–217.
WeberK., and OsbornM. (1969).J. Biol. Chem. 244, 4406–4412.
YancopoulosG. D., and AltF. W. (1986).Annu. Rev. Immunol. 4, 339–368.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pazur, J.H., Tay, M.E., Pazur, B.A. et al. Observations of a set of isomeric anti-lactose antibodies directed against a group D streptococcal polysaccharide. J Protein Chem 6, 387–399 (1987). https://doi.org/10.1007/BF02343337
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02343337