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Immunogenetics

, Volume 29, Issue 1, pp 6–13 | Cite as

Regulation of expression of mouseC4 andSlp genes by non-H-2-linked genes

  • Sylvia M. Bruisten
  • Peter Demant
Article

Abstract

C4 (the fourth complement component) and Slp (sexlimited protein) are two homologous plasma proteins encoded by genes in theS-region of theH-2 gene complex. We studied the genetic factors influencing the plasma levels of these proteins and their mRNA levels in liver. Considerable differences in both protein and mRNA levels were found between mouse strains carrying the sameS-region allele on different genetic backgrounds, indicating a pretranslational effect of non-H-2-linked genes on the expression of the twoS-region genes. The expression of Slp is androgen-dependent in the strains tested. However, testosterone treatment cannot increase the low levels of Slp caused by non-H-2-linked regulatory genes. In mice with Slp-negativeS-region alleles we found liver mRNA hybridizing with Slp-specific oligonucleotides, indicating expression of theSlp gene in Slp-negative strains. Our data demonstrate the complexity of the regulation of theC4 andSlp genes and pave the way for the analysis of the regulatory factors involved.

Keywords

mRNA Level Testosterone Plasma Level Regulatory Gene Genetic Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Sylvia M. Bruisten
    • 1
  • Peter Demant
    • 1
  1. 1.Division of Molecular GeneticsThe Netherlands Cancer Institute (Antoni van Leeuwenhoekhuis)CX AmsterdamThe Netherlands

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