International Journal of Legal Medicine

, Volume 105, Issue 2, pp 75–80 | Cite as

ABH-related antigens in human male genital tract

A histochemical examination
  • Katsuji Nishi
  • Tatsushige Fukunaga
  • Yoshio Yamamoto
  • Mitsuko Yamada
  • Masateru Kane
  • Akio Tanegashima
  • Steven Rande
  • Bernd Brinkmann
Original Articles

Summary

The localization of ABH related antigens in human male reproductive tract was examined using monoclonal antibodies and an avidin biotin complex method. No positive reaction with blood group antibodies on spermatozoa was observed in testis and ductus epididymidis apart from erythrocytes and endothelial cells. The expression of ABH and ABH related antigens in ductuli efferentes testis, ductus epididymidis, seminal vesicle and prostate was complexly coded by a combination of H, Se, Le and X genes. The results obtained in this study indicate that the ABH antigens detected on spermatozoa of seminal stains are coating antigens and not inherent to the cell membrane, and the ABO, H, Se, Le and X genes are subjected to a tissue-dependent differential expression.

Key words

Immunohistochemistry ABH-related antigens Human male genital tract 

Zusammenfassung

Die Lokalisation (and Verteilung) der Antigene des ABH-Komplexes im Bereich der inneren Geschlechtsorgane des Mannes wurde mittels monoklonaler Antikörper unter Benutzung einer Avidin-Biotin Technik untersucht. Dabei konnten positive Reaktionen im Hoden und im Ductus epididymidis lediglich an Erythrozyten und Endothelzellen beobachtet werden. Die Expression von ABH-Antigenen in den Ductuli efferentes testis, im Ductus epididymidis, in den Samenbläschen und der Prostata wird offensichtlich komplex durch H-, Se-, Le- und X-Gene kodiert. Die Resultate der vorliegenden Untersuchungen zeigen, daß die ABH-Antigene der Spermienoberfläche offensichtlich aus der Samenflüssigkeit stammen und die ABO-, H-, Se-, Le-und X-Gene gewebsabhängig unterschiedlich exprimiert werden.

Schlüsselwörter

Immunohistochemistry Antigene des ABH-Komplexes Männlicher Genitaltrakt 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Katsuji Nishi
    • 1
  • Tatsushige Fukunaga
    • 1
  • Yoshio Yamamoto
    • 1
  • Mitsuko Yamada
    • 1
  • Masateru Kane
    • 1
  • Akio Tanegashima
    • 1
  • Steven Rande
    • 2
  • Bernd Brinkmann
    • 2
  1. 1.Department of Legal MedicineShiga University of Medical ScienceOhtsuJapan
  2. 2.Institut für RechtsmedizinMünster UniversitätMünsterGermany

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