Journal of Molecular Evolution

, Volume 43, Issue 3, pp 312–314 | Cite as

A new transmembrane 4 superfamily molecule in the nematode,Caenorhabditis elegans

  • Michael G. Tomlinson
  • Mark D. Wright
Letter to the Editor


Transmembrane 4 superfamily (TM4SF) molecules are predominantly mammalian cell surface glycoproteins that are thought to transduce signals mediating cell development, activation, and motility. Analysis of the Genpept sequence database reveals YKK8, a novel member of the TM4SF in the nematode,Caenorhabditis elegans. YKK8 is a putative 27.4-kDa protein encoded by a gene on chromosome III of theC. elegans genome (Wilson et al. [1994]Nature 368:32–38). The assignment of YKK8 to the TM4SF is justified by three criteria: statistical comparison of protein sequences, conserved TM4SF protein sequence motifs, and conserved TM4SF intron/exon boundaries in the genomic sequence. The discovery of a TM4SF molecule in the nematode extends this superfamily to a more primitive branch of the phylogenetic tree and suggests a fundamental role for TM4SF molecules in biology.

Key words

Transmembrane 4 superfamily YKK8 Caenorhabditis elegans 


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  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410CrossRefPubMedGoogle Scholar
  2. Barclay AN, Birkeland ML, Brown MH, Beyers AD, Davis SJ, Somoza C, Williams AF (1993) The leucocyte antigen factsbook. Academic Press, LondonGoogle Scholar
  3. Behr S, Schriever F (1995) Engaging CD19 or Target of an Anti-proliferative Antibody 1 on human B lymphocytes induces binding of B cells to the interfollicular stroma of human tonsils via integrin α4/β1 and fibronectin. J Exp Med 182:1191–1199CrossRefPubMedGoogle Scholar
  4. Boismenu R, Rhein M, Fischer WH, Havran WL (1996) A role for CD81 in early T cell development. Science 271:198–200PubMedGoogle Scholar
  5. Dayhoff MO, Barker WC, Hunt LT (1983) Establishing homologies in protein sequences. Methods Enzymol 91:524–545PubMedGoogle Scholar
  6. Devereux J, Harberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–407PubMedGoogle Scholar
  7. Dong J-T, Lamb PW, Rinker-Schaeffer CW, Vukanovic J, Ichikawa T, Isaacs JT, Barrett JC (1995)KAII, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268:884–886PubMedGoogle Scholar
  8. Fitter S, Tetaz TJ, Berndt MC, Ashman LK (1995) Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3. Blood 86:1348–1355PubMedGoogle Scholar
  9. Levy S, Nguyen VQ, Andria ML, Takahashi S (1991) Structure and membrane topology of TAPA-1. J Biol Chem 266:14597–14602PubMedGoogle Scholar
  10. Tomlinson MG, Williams AF, Wright MD (1993) Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the transmembrane 4 superfamily. Eur J Immunol 23:136–140PubMedGoogle Scholar
  11. Wilson R, Ainscough R Anderson K, Baynes C, Berks M, Bonfield J, Burton J, Connell M, Copsey T, Cooper J, Coulson A, Craxton M, Dear S, Du Z, Durbin R, Favello A, Fraser A, Fulton L, Gardner A, Green P, Hawkins T, Hillier L, Jier M, Johnston L, Jones M, Kershaw J, Kirsten J, Laisster N, Latreille P, Lightning J, Lloyd C, Mortimore B, O'Callaghan M, Parsons J, Percy C, Rifken L, Roopra A, Saunders D, Shownkeen R, Sims M, Smaldon N, Smith A, Smith M, Sonnhammer E, Staden R, Sulston J, Thieny-Mieg J, Thomas K, Vaudin M, Vaughan K, Waterston R, Watson A, Weinstock L, Wilkinson-Sproat J, Wohldman P (1994) 2.2 Mb of contiguous nucleotide sequence from chromosome III ofC. elegans. Nature 368:32–38PubMedGoogle Scholar
  12. Wright MD, Tomlinson MG (1994) The ins and outs of the transmembrane 4 superfamily. Immunol Today 15:588–594CrossRefPubMedGoogle Scholar
  13. Wright MD, Rochelle JM, Tomlinson MG, Seldin MF, Williams AF (1993) Gene structure, chromosomal location and protein sequence of mouse CD53: evidence that the transmembrane 4 superfamily arose by gene duplication. Int Immunol 5:209–216PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1996

Authors and Affiliations

  • Michael G. Tomlinson
    • 1
  • Mark D. Wright
    • 2
  1. 1.MRC Cellular Immunology Unit, Sir William Dunn School of PathologyUniversity of OxfordOxfordEngland
  2. 2.The Walter and Eliza Hall Institute of Medical ResearchPost Office RMHVictoriaAustralia

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