Actin gene structure in twoArtemia species,A. franciscana andA. parthenogenetica
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Genomic clones coding for actin have been isolated from two species of the crustaceanArtemia,A. parthenogenetica andA. franciscana. The Act211 isoform gene was isolated from A.parthenogenetica, and the two other isoform genes, Act302 and Act403, were isolated fromA. franciscana. The comparison of the nucleotide sequence of genomic and cDNA clones showed an interspecific divergence of 4% in translated and 6.1% in untranslated regions. However, the establishment of the partial structure of the Act211 gene inA. franciscana and of the Act302 gene inA. parthenogenetica suggests their similarity in the two species. The Act211 gene is divided into four exons, the Act302 gene into six exons, and the Act403 gene into seven exons. The three genes have introns in the 5′ untranslated region and between codons 41 and 42. The Act211 and 403 genes have one common intron in codon 168. The Act302 and 403 genes have common introns between codons 121–122, 246–247, and within codon 301. While introns in the 5′ untranslated region and between codons 41–42 and 121–122 are present in many organisms, the introns in positions 168 and 246–247 had only been found previously in actin genes from the nematodeOnchocerca volvulus and the green algaVolvox carterii, respectively. The intron in position 301 had not been reported before. The transcription initiation sites of these three genes as well as the nucleotide sequences of the promoter regions have been also determined.
KeywordsActin Artemia Crustaceans Exon Gene structure Intron Promoter
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- Browne RA, Sorgeloos P, Trotman CNA (1991) in Artemia biology. CRC Press, BostonGoogle Scholar
- Dorit RL, Ohara O (1993) cDNA amplification using one-sided (anchored) PCR. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Strobl K (eds) Current protocols in molecular biology. John Wiley and Sons, New York, pp 15.6.1–15.6.10Google Scholar
- Hennessey ES, Drummond DR, Sparrow JC (1993) Molecular genetics of actin function. Biochem J 282:657–671Google Scholar
- Jones N (1994) Transcriptional regulation by dimerization: two sides to an incestuous relationship. Cell 61:9–11Google Scholar
- Kasturi KN, Bona CA (1994) Analysis of the expression immunoglobulin gene repertoire by screening libraries derived from PCR-amplified cDNA. Nucleic Acids Res 19:6339–6340Google Scholar
- Mounier N, Prudhomme JC (1991) Differential expression of muscle and cytoplasmic actin genes during development of Bombyx mori. Insect Biochem 21:523–533Google Scholar
- Ortega MA, Macias MT, Martinez J-L, Palmero I, Sastre L (1992) Expression of actin isoforms inArtemia. In: El Haj A (ed) Molecular biology of muscle. The Company of Biologists, Cambridge, pp 131–137Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
- Sheterline P, Sparrow JC (1994) Protein profile: actin. Academic Press, LondonGoogle Scholar
- Triezenberg SJ (1993) Primer extension. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. John Wiley and Sons, New York, pp 4.8.1–4.8.5Google Scholar