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Journal of Comparative Physiology B

, Volume 166, Issue 7, pp 412–417 | Cite as

Ca2+ sensitivity and caffeine-induced changes in skinned cardiac muscle fibers of the carp,Cyprinus carpio

  • A. Chugun
  • K. Temma
  • H. Kondo
  • N. Kurebayashi
Original Paper

Abstract

Ca2+ sensitivity and caffeine-induced sensitivity changes in skinned carp heart fibers were compared with those of guinea pig and rat heart. The Ca2+ concentration-response curves of saponin-treated left atrial skinned fibers obtained from guinea pig and rat were almost identical. Doses of 5 and 20 mmol·l-1 caffeine shifted this curve to the left. However, when a relatively high concentration (50 mmol·l-1) of caffeine was used, the left-ward shift was reduced. Caffeine reduced the peak of the Ca2+ concentration-response curve. The Ca2+ concentration-response curve of carp atrial skinned fiber is almost identical to that of guinea pig and rat. However, a further increase in Ca2+ sensitivity was observed even when 50 mmol·l-1 caffeine was added. Similarly, a decrease in the response curve peak was also observed. Ca2+ sensitivity in ventricular skinned fibers obtained from carp was almost the same as that observed for the atrial, but the increase in Ca2+ sensitivity due to caffeine was larger In addition, a further increase was also observed when 50 mmol·l-1 caffeine was added. These results indicate that the Ca2+ sensitivity of contractile proteins in atrial muscles from carp heart is the same as that of guinea pig and rat. It is, however, assumed that there are some differences in properties in the contractile proteins. It is also assumed that there are some differences between the atrial and ventricular muscles of carp heart.

Key words

Carp Heart Ca2+ sensitivity Skinned fibers Caffeine 

Abbreviations

ED50

Ca2+ concentration which causes half maximal effects

EGTA

ethylene glycol bis (β-aminoethylether)-N,N,N′,N′-tetraacetic acid

HEPES

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

MOPS

3-(N-Morpholino)-propanesulfonic acid

pCa

log10[free Ca2+]

SR

sarcoplasmic reticulum

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References

  1. Baudet S, Ventura-Clapier R (1990) Differential effects of caffeine on skinned fibers from control and hypertrophied ferret hearts. Am J Physiol 259: H1803-H1808PubMedGoogle Scholar
  2. Bers DM (1985) Ca2+ influx and sarcoplasmic reticulum Ca2+ release in cardiac muscle activation during postrest recovery. Am J Physiol 248: H366-H381PubMedGoogle Scholar
  3. De Beer EL, Grundeman RLF, Wilhelm AJ, Caljouw CJ, Klepper D, Schiereck P (1988) Caffeine suppresses length dependency of Ca2+ sensitivity of skinned striated muscle. Am J Physiol 254: C491-C497PubMedGoogle Scholar
  4. Driedzič WR, Gesser H (1988) Differences in force-frequency relationships and calcium dependency between elasmobranch and teleost heart. J Exp Biol 140: 227–241Google Scholar
  5. Fabiato A (1981) Effects of cyclic AMP and phosphodiesterase inhibitors on the contractile activation and the Ca2+ transient detected with aequorin in skinned cardiac cells from rat and rabbit ventricles. J Gen Physiol 78: 15a-16aCrossRefGoogle Scholar
  6. Fabiato A (1982) Calcium release in skinned cardiac cells: variations with species tissues and development. Fed Proc 41: 2238–2244PubMedGoogle Scholar
  7. Fabiato A, Fabiato F (1978) Cyclic AMP-induced ehnancement of calcium accumulation by the sarcoplasmic reticulum with no modification of the sensitivity of the myofilaments to calcium in skinned fibers from a fast skeletal muscle. Biochim Biophys Acta 539: 253–260PubMedGoogle Scholar
  8. Goldstein A, Aronow L, Kalman S (1974) Molecular mechanisms of drug action. In: Goldstein A et al. (eds) Principles of drug action. Wiley, New York, pp 1–128Google Scholar
  9. Gwathmey JK, Hajjar RJ (1992) Calcium activated force in a Turkey model of spontaneous dilated cardiomyopathy: adaptive changes in thin myofilament Ca2+ regulation with resultant implications on contractile performance. J Mol Cell Cardiol 24: 1459–1470CrossRefPubMedGoogle Scholar
  10. Harrison SM, Bers DM (1990) Temperature dependence of myofilament Ca2+ sensitivity of rat, guinea-pig and frog ventricular muscle. Am J Physiol C274–C281Google Scholar
  11. Khandoudi N, Guo AC, Chesnais M, Feuvray D (1993) Skinned cardiac fibers of diabetic rats: contractile activation and effects of 2,3-butanedione monoxime (BDM) and caffeine. Cardiovasc Res 27: 453–458Google Scholar
  12. Kitazawa T (1988) Caffeine contracture in guinea-pig ventricular muscle and the effect of extracellular sodium ions. J Physiol (Lond) 420: 703–729Google Scholar
  13. Kurebayashi N, Ogawa Y (1988) Increase by trifluoperazine calcium sensitivity of myofibrils in a skinned fiber from frog skeletal muscle. J Physiol (Lond) 403: 407–424Google Scholar
  14. Langer GA, Brady AJ, Tan ST, Serena SD (1975) Correlation of the glycoside response the force staircase and the action potential configuration in the neonatal rat heart. Circ Res 36: 744–752PubMedGoogle Scholar
  15. Morgan JP, Blinks JR (1982) Intercellular Ca2+ transients in the cat papillary muscle Can J Physiol Pharmacol 60: 524–528PubMedGoogle Scholar
  16. Reuter R (1974) Exchange of calcium ions in the mammalian myocardium. Circ Res 34: 599–605PubMedGoogle Scholar
  17. Saito T, Temma K (1976) Effects of left and right vagal stimulation on excitation and conduction of the carp heart (Cyprinus carpio). J Comp Physiol 111 39–53Google Scholar
  18. Santer RM (1974) The organization of the sarcoplasmic reticulum in teleost ventricular myocardial cells. Cell Tissue Res 151: 395–402CrossRefPubMedGoogle Scholar
  19. Santer RM, Cobb JLS (1972) The fine structure of the heart of the teleostPleuronectes platessa. Z. Zellforch 131: 1–14Google Scholar
  20. Steele DS, Smith GL (1992) The effects of caffeine and Ca2+ on rigor tension in triton-treated rat ventricular trabeculae. Pflügers Arch 421: 343–349CrossRefPubMedGoogle Scholar
  21. Stemmer P, Akera T (1986) Concealed positive force-frequency relationships in rat and mouse cardiac muscle revealed by ryanodine. Am J Physiol 251: H1106–1110PubMedGoogle Scholar
  22. Temma K, Nagatomi H, Hirano H, Kitazawa T, Kondo H (1987) Carp (Cyprinus carpio) heart has a high sensitivity to the positive inotropic effect of strophanthidin despite negative force-frequency relationships. Gen Pharmacol 18: 617–622PubMedGoogle Scholar
  23. Tibbits GF, Hove-Madsen L, Bers DM (1991) Calcium transport and the regulation of cardiac contractility in teleosts: a comparison with higher vertebrates. Can J Zool 69: 2014–2019Google Scholar
  24. Wendt IR, Stephenson DG (1983) Effects of caffeine on Ca2+-activated force production in skinned cardiac and skeletal muscle fibers of the rat. Pflügers Arch 398: 210–216CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • A. Chugun
    • 1
  • K. Temma
    • 2
  • H. Kondo
    • 1
  • N. Kurebayashi
    • 3
  1. 1.Department of Veterinary Pharmacology, School of Veterinary Medicine and Animal SciencesKitasato UniversityTowada-shi, AomoriJapan
  2. 2.Department of Toxicology, School of Veterinary Medicine and Animal SciencesKitasato UniversityTowada-shi, AomoriJapan
  3. 3.Department of Pharmacology, School of MedicineJuntendo UniversityTokyoJapan

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