Detecting immunoglobulin abnormalities in the cerebrospinal fluid (CSF): Which methods are best for clinical purposes?

  • Laurenzi M. A. 
  • Provinciali L. 
  • Giovagnoli A. R. 
  • Busco
  • Bocchini V. 
  • Paris L. 
  • Chiodi F. 
Original Articles


Several techniques are now available for the quantitative and qualitative examination of CSF proteins modified during the course of neurological disease. The CSF and serum of 42 patients suffering from various neurological diseases and of 7 controls were examined using quantitative methods-single radial immunodiffusion, nephelometric analysis-and qualitative methods—(i) isoelectric-focusing on polyacrylamide gel and (ii) on agarose gel, isoelectric focusing of unconcentrated CSF followed by transfer to cellulose nitrate membranes and (iii) immunoperoxidase staining and (iv) double antibody peroxidase labeling and avidin-biotin amplification. In the present study the results and the advantages of different methods are described and compared.


CSF immunoglobulins quantitative and qualitative methods single radial immunodiffusion nephelometric analysis-isoelectric focusing on polyaerylamide and agarose gelimmunoperoxidase staining avidin-biotin amplification 


Numerose metodiche sono attualmente disponibili per l'esame quantitativo e qualitativo delle proteine liquorali che risultano alterate in numerose malattie neurologiche.

Liquor e siero di 42 pazienti affetti da diverse malattie neurologiche e di 7 controlli sono stati esaminati con metodiche quantitative — immunodiffusione radiale, analisi nefelometrica — e qualitative — (i) isoelettro focusing su poliacrilamide e (ii) su agarosio, isoelettrofocusing di liquor non concentrato seguito da trasferimento su membrane di nitrato di cellulosa e (iii) colorazione con immunoperossidasi e (iv) doppia definizione con perossidasi ed anticorpi seguito da amplificazione con avidin-biotina.

I risultati ed i vantaggi di ogni metodica sono descritti e confrontati tra loro.


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Copyright information

© Masson Italia Editori 1987

Authors and Affiliations

  • Laurenzi M. A. 
    • 1
  • Provinciali L. 
    • 1
  • Giovagnoli A. R. 
    • 1
  • Busco
    • 2
  • Bocchini V. 
    • 3
  • Paris L. 
    • 4
  • Chiodi F. 
    • 4
  1. 1.Istituto Malattie del Sistema NervosoUniversità di AnconaItaly
  2. 2.Laboratorio AnalisiUSL n. 12AnconaItaly
  3. 3.Istituto di Biologia GeneraleUniversità di PerugiaItaly
  4. 4.Clinica Neurologica IIIUniversità di RomaItaly

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