Abstract
A highly efficient new method for the generation of antigen specific T cell lines (TCL) is now available. By this method we established 134 myelin basic protein (MBP) TCL from the peripheral blood of 9 patients with definite multiple sclerosis (n=69) and 8 healthy donors (n=65). The yield of MBP reactive TCL in the two groups was comparable.
So far 22 MBP specific TCL from 7 patients and 24 from 7 healthy individuals have been tested for their proliferative response to a panel of four synthetic peptides representing MBP residues 7–26, 80–99, 139–153 and 148–162. Although the peptide sequences did not encompass the whole MBP, the pattern of reactivity to these peptides in patients and controls seems to be similar. Further, when multiple TCL from the same donor were analysed, no dominant recognition emerged.
Sommario
È attualmente disponibile una nuova metodica per ottenere linee T linfocitarie antigene-specifiche con notevole efficienza. Utilizzando tale approccio abbiamo ottenuto 134 linee MBP specifiche dal sangue di 9 pazienti con MS (n=69) e 8 soggetti sani (n=65). L'efficienza con cui sono state ottenute tali linee è risultata comparabile fra i due gruppi.
Abbiamo poi testato la risposta a 4 peptidi della MBP (residui 7–27, 80–99, 139–153 e 148–162) da parte di 22 linee di 6 pazienti e 24 linee di 7 sani.
Sebbene i peptidi impiegati non coprissero l'intera sequenza della MBP, il profilo di risposta ad essi è apparso sovrapponibile nei pazienti e nei controlli.
Se si analizzano diverse linee provenienti dallo stesso soggetto, non risulta evidente alcun riconoscimento di epitopi dominanti.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albert E., Bauer M.P., Mayer W.R., eds.:Histocompatibility testing 1984. Berlin: Springer Verlag, 1984.
Allegretta M., Nicklas J.A., Sriram S. et al:T cells responsive to Myelin Basic Protein in patients with Multiple Sclerosis. Science 247:718–721, 1990.
Alvord E.C., Kies M.W., Suckling A.J., eds.:Experimental Allergic Encephalomyelitis: a useful model for Multiple Sclerosis. New York: Alan R Liss, 1984.
Confavreux C., Aimard G., Devic M.:Course and prognosis of Multiple Sclerosis assessed by the computerized data processing of 349 patients. Brain 103:281–300, 1980.
Eylar E.H., Kniskern P.J., Jackson J.J.:Myelin Basic Proteins. Methods Enzymol. 32:323–341, 1979.
Hafler D.A., Weiner H.L.:MS: a CNS and systemic autoimmune disease. Immunol. Today 10:104–107, 1989.
Martin R., Jaraquemada D., Flerlage M. et al.:Fine specificity and HLA restriction of Myelin Basic Protein-specific cytotoxic T cell lines from Multiple Sclerosis and healthy individuals. J. Immunol. 145:540–548, 1990.
Martin R., Howell M.D., Jaraquemada D. et al:A Myelin Basic Protein peptide is recognized by cytotoxic T cells in the context of four HLA-DR types associated with Multiple Sclerosis. J. Exp. Med. 173:19–24, 1991.
Olsson T., Zhi W.W., Hojeberg B. et al.:Autoreactive T lymphocytes in Multiple Sclerosis determined by antigen-induced secretion of interferon. J. Clin. Invest. 86:981–984, 1990.
Ota K., Matsui M., Milford E.L. et al.:T cell recognition of an immunodominant Myelin Basic Protein epitope in Multiple Sclerosis. Nature 346:183–187, 1990.
Pette M., Fujita K., Wilkinson D. et al.:Myelin autoreactivity in Multiple Sclerosis: recognition of Myelin Basic Protein in the context of HLADR2 products by T lymphocytes of Multiple Sclerosis patients and healthy donors. Proc. Natl. Acad. Sci. 87:7986–7972, 1990.
Pette M., Fujita K., Kitze B. et al.:Myelin Basic Protein-specific T lymphocyte lines from MS patients and healthy individuals. Neurology 40:1770–1776, 1990.
Schumacher G.A., Beebe G., Kibler R.F. et al.:Problems of experimental trials of therapy in Multiple Sclerosis: report by the panel on the evaluation of experimental trials of therapy in Multiple Sclerosis. Ann. NY Acad. Sci. 122:552–568, 1965.
Wucherpfennig K.W., Ota K., Endo N. et al.:Shared human T cell receptor V usage to immunodominant regions of Myelin Basic Protein. Science 248:1016–1019, 1990.
Author information
Authors and Affiliations
Additional information
Paper presented at the National Congress at Sorrento in 1991 and selected by the Editorial Board of the Journal
Rights and permissions
About this article
Cite this article
Ristori, G., Salvetti, M., Buttinelli, C. et al. Target epitopes of myelin basic protein specific T cell lines in multiple sclerosis. Ital J Neuro Sci 14, 139–143 (1993). https://doi.org/10.1007/BF02335748
Issue Date:
DOI: https://doi.org/10.1007/BF02335748