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Experimental allergic encephalomyelitis in the monkey: humoral immunity and blood-brain barrier function

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Abstract

Acute monophasic experimental autoimmune encephalomyelitis (AMEAE) was induced in 5 Macacus cynomolgus monkeys. Serum and cerebrospinal fluid (CSF) paired samples were colleced before and four weeks after immunization, time of complete development of AMEAE clinical picture. After immunization the CSF/serum albumin ratio and the IgG index were markedly increased. Agerose isoelectric focusing (AIEF) followed by immunofixation revealed faint IgG oligoclonal bands in both serum and CSF of all monkeys, which became more evident after Kappa and Lambda assessment. Intrathecal synthesis of IgG oligoclonal bands was detected in only one CSF. Affinity-driven immunoblotting failed to detect anti-myelin basic protein (MBP) oligoclonal IgG. No free light chain (FLC) patterns or IgA and IgM oligoclonal bands were detected.

Sommario

Gli Autori hanno indotto una encefalomielite autoimmune sperimentale acuta monofasica in 5 scimmie (Macacus cynomolgus).

Campioni di liquido cerebrospinale (LCS) e del rispettivo siero sono stati prelevati prima e quattro settimane dopo l'immunizzazione, tempo necessario per il completo manifestarsi del quadro clinico. Dopo l'immunizzazione il rapporto dell'albumina LCS/siero e l'IgG index erano aumentati in notevole misura. La focalizzazione isoelettrica in agarosio, seguita da doppia immunofissazione, amplificazione con avidina-biotina e colorazione con perossidasi, ha evidenziato bande oligoclonali (BO) IgG sia nel LCS che nel siero di tutte le scimmie esaminate, tuttavia il pattern oligoclonale appariva più evidente dopo immunofissazione contro le catene leggere. La sintesi intratecale di BO IgG è stata evidenziata solo in un campione di LCS. L'immunoblotting per affinità non ha permesso di evidenziare BO IgG dirette contro la proteina basica della mielina. Non sono state evidenziate bande oligoclonali IgA e IgM, come non sono state evidenziate catene leggere libere.

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Authors and Affiliations

  1. Clinica Neurologica, Università di Padova, Via Giustiniani 5, 35128, Padova, Italy

    Gallo P., Bracco F., Tavolato B. & Battistin L.

  2. Institute of Pathological Physiology, University of Belgrade, Yugoslavia

    Cupic D. & Krzalic L.

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  1. Gallo P.
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  2. Cupic D.
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  3. Bracco F.
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  4. Krzalic L.
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  5. Tavolato B.
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  6. Battistin L.
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Gallo, P., Cupic, D., Bracco, F. et al. Experimental allergic encephalomyelitis in the monkey: humoral immunity and blood-brain barrier function. Ital J Neuro Sci 10, 561–565 (1989). https://doi.org/10.1007/BF02333791

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