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Zeitschrift für Rechtsmedizin

, Volume 83, Issue 3, pp 225–232 | Cite as

Investigation of the Short-Time Autolysis of Rat Hearts by Means of SDS

Polyacrylamide gel electrophoresis and electron microscopy
  • E. Vándor
  • T. Varga
Originalarbeiten / Original Works

Summary

The short-time autolysis of hearts was regarded as a model of ischaemic heart failure. Therefore, isolated rat hearts were subjected to 30–120 min autolysis in a Locke solution at 37°C. Electron microscopic examinations and myofibrillar preparations were made from the autolysed heart ventricles. The myofibrillar proteins were resolved by SDS-polyacrylamide gel electrophoresis. After 30 min autolysis the amount of a protein of 192,000 daltons greatly increased. At the same time on the electron micrographs the focal destruction of filaments adhering to intercalary discs could be observed as well as a focal filament destruction on the A filament area and the mitochondrial structure altered too. After 60 min autolysis another protein of 36,400 daltons appeared. On the electron micrographs the focal desintegration of Z membranes and the focal destruction of I filaments can be observed. After 120 min autolysis further proteolytic products could not be detected by gel electrophoresis but on the electron micrographs the destruction of Z membranes and I filaments became more pronounced.

Key words

Autolysis, rat heart Ischaemia, short-time autolysis as a model 

Zusammenfassung

Die Kurzzeitautolyse des Myocard wird als Beispiel für einen Hypoxieschaden betrachtet. Isolierte Rattenherzen wurden einer Autolyse von 30, 60 und 120 min in Lockescher Lösung bei 37°C unterworfen und elektronenmikroskopische Untersuchungen sowie eine Präparation der Myofibrillen durchgeführt. Die Proteine der Myofibrillen wurden durch SDS- Polyacrylamid-Gel-Elektrophorese aufgetrennt. Nach 30 min Autolyse fand sich eine Zunahme eines Proteins von 192000 Dalton. Zur gleichen Zeit konnte elektronenmikroskopisch sowohl eine fokale Zerstörung der Filamente, die zu den Zwischenscheiben gehören, als auch die fokale Zerstörung der Filamente im Bereich der A-Filamente beobachtet werden. Auch änderte sich die Mitochondrienstruktur. Nach 60 min Autolyse erschien ein weiteres Protein von 36 400 Dalton. Elektronenmikroskopisch konnte eine fokale Desintegration der Z-Membranen und eine fokale Zerstörung der I-Filamente beobachtet werden. Nach 120 min Autolyse fanden sich elektrophoretisch keine weiteren proteolytischen Produkte, aber elektronenmikroskopisch zeigte sich eine Zerstö rung der Z-Membranen und I-Filamente.

Schlüsselwörter

Autolyse, Rattenmyocard Hypoxie, Kurzzeitautolyse als Beispiel 

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Copyright information

© Springer-Verlag Berlin • Heidelberg 1979

Authors and Affiliations

  • E. Vándor
    • 1
  • T. Varga
    • 2
  1. 1.National Institute of TraumatologyBudapestHungary
  2. 2.Institute of Experts in Forensic MedicineBudapestHungary

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