Journal of Assisted Reproduction and Genetics

, Volume 11, Issue 3, pp 162–164 | Cite as

Effect of preincubation of human spermatozoa in milk on penetration of zona-free hamster oocytes: Comparison to TEST yolk

  • M. Joseph Scobey
  • Wendy J. Holmgren
  • Rajasingam S. Jeyendran
Animal Investigations



To investigate the effect of preincubation of human sperm in milk on their ability to penetrate zona-free hamster oocytes, 27 ejaculates were studied. Each ejaculate was divided into two portions and incubated in either milk or TEST yolk (TESTY) for 22–24 hr at 5‡C prior to processing for the sperm penetration assay.


Spermatozoa preincubated in milk penetrated a significantly (P<0.05) higher percentage of oocytes (mean±SE; 74.4±6.3) than spermatozoa preincubated in TESTY (66.8±6.6). Statistically, no significant differences were noted for penetration index or postincubation sperm motility between the two media.


Preincubation of sperm in milk therefore appears to enhance their ability to penetrate zona-free hamster oocytes, compared to TESTY preincubation. Since milk is natural, simple, inexpensive, readily available, and can be easily processed, it should be the medium of choice for sperm preincubation.

Key words

human spermatozoa milk sperm penetration assay TEST yolk 


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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • M. Joseph Scobey
    • 1
  • Wendy J. Holmgren
    • 1
  • Rajasingam S. Jeyendran
    • 1
  1. 1.Department of Obstetrics and Gynecology, Prentice Women's HospitalNorthwestern University Medical SchoolChicago

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