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Laminins, tenascin and type VII collagen in colorectal mucosa

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Summary

The distribution of different laminin polypeptides, type VII collagen and tenascin has been studied in adult and foetal colorectal mucosa by using the indirect immunofluorescence technique. Immunoreactivity for laminin α1 chain was located to basement membranes of epithelia, muscularis mucosae, and blood vessels, respectively in different segments of adult colon and rectum. Laminin β1 and γ1 chains were additionally expressed in lamina propria. Laminin α2 chain was also found in lamina propria around the pericyptal fibrollasts. Immunoreactivity for laminin β2 chain was restricted to basement membranes in the muscularis mucosae and arteries. Laminin α3 and β3 chains, suggestive for laminin-5, were confined especially to surface epithelial basement membranes. Immunoreactivity for type VII collagen was confined to basement membrane of surface epithelium in a punctate manner, while that for tenascin was seen slightly more broadly in the basement membrane zone and also in the muscular layer. The distribution of laminin chains in 16-week-foetal colon mostly resembled that of corresponding adult tissue, although immunoreactivities for laminin α2 and β2 chains were lacking. Type VII collagen and the high molecular weight isoform of tenascin also absent from the foetal colon. The results show that the basement membrane of the surface epithelium of colon and rectum express the components of epithelial adhesion complex, laminin-5 (α3-β3-γ2) and type VII collagen, resembling in this respect small intestine and stomach while laminin-2 (α2-β1-γ1) appears to be associated with pericryptal fibroblasts, and laminin-1 (α1-β1-γ1) widely in most basement membranes.

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Authors and Affiliations

  1. Department of Anatomy, Institute of Biomedicine, (Siltavuorenpenger 20A), P.O. Box 9, Finland

    Jouni Lohi, Taneli Tani & Ismo Virtanen

  2. Department of Pathology, University of Helsinki, (Haartmaninkatu 3), P.O. Box 21, FIN-00014, Finland

    Ilmo Leivo

  3. Second Department of Surgery, Helsinki University Central Hospital, Haartmaninkatu 2, FIN-00290, Helsinki, Finland

    Tuula Kiviluoto & Eero Kivilaakso

  4. Cultaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th Street, 02129, Charlestown, MA, USA

    Robert E. Burgeson

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  1. Jouni Lohi
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  2. Ilmo Leivo
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Lohi, J., Leivo, I., Tani, T. et al. Laminins, tenascin and type VII collagen in colorectal mucosa. Histochem J 28, 431–440 (1996). https://doi.org/10.1007/BF02331434

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