Summary
To quantitate the developmental changes in selenium-dependent cellular glutathione peroxidase during the perinatal period, tissue sections from foetal (day 12 to day 22) and neonatal (day 6) rats were stained immunohistochemically using specific polyclonal antiserum. The intensity of the staining was quantified by fluorescence microscopy image analysis. There was a general trend of enriched glutathione peroxidase in the epithelial linings and metabolically active sites. Significant fluorescence was detected in cardiomyocytes, hepatocytes, renal tubular epithelium, bronchiolar epithelium and intestinal epithelium at day 15. The intensity increased in a stepwise manner therafter. The overall increase in the intensity of staining in the heart, liver, kidneys, lungs and intestine was 1.5-, 2.3-, 1.6-, 1.7- and 3.0-fold, respectively. The phase of most rapid increase occurred during the foetal period in the liver, intestine and heart. In the kidneys and lungs, glutathione peroxidase increased significantly during foetal life, and to a similar extent postnatally. These results suggest that the intracellular H2O2-scavenging system develops during the foetal period as an essential mechanism for living under atmospheric oxygen conditions. The late development observed in the kidneys and lungs is consistent with the relative biological immaturity of these organs in full-term neonates.
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Asayama, K., Hayashibe, H., Dobashi, K., Uchida, N., Kobayashi, M., Jawaoi, A. &Kato, K. (1991) Immunohistochemical study on prenatal development of rat superoxide dismutases in lungs and kidneys.Pediatr. Res. 29, 487–91.
Asayama, K., Yokota, S., Dobashi, K., Hayashibe, H., Kawaoi, A. &Nakazawa, S. (1994) Purification and immunoelectronmicroscopic localization of cellular glutathione peroxidase in rat hepatocytes: Quantitative analysis by post-embedding method.Histochemistry 102, 213–19.
Chen, Y. &Frank, L. (1993) Differential gene expression of antioxidant enzymes in the perinatal rat lung.Pediatr. Res. 34, 27–31.
Chu, F. F., Esworthy, R. S., Doroshow, J. H., Doan, K. &Liu, X. F. (1992) Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents.Blood 79, 3233–8.
Chu, F. F., Doroshow, J. H. &Esworthy, R. S. (1993) Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSH-PxGI.J. Biol. Chem. 268, 2571–8.
Coursin, D. B., Cihla, H. P., Oberley, T. D. &Oberley, L. W. (1992) Immunolocalization of antioxidant enzymes and isoenzymes of glutathione S-transferase in normal rat lung.Am. J. Physiol. 263, L679-L691.
De Haan, J. B., Tymms, M. J., Cristiano, F. &Kola, I. (1994) Expression of copper/zinc superoxide dismutase and glutathione peroxidase in organs of developing mouse embryos, foetuses, and neonates.Pediatr. Res. 35, 188–96.
Dobashi, K., Asayama, K., Hayashibe, H., Uchida, N., Kobayashi, M., Kawaoi, A. &Kato, K. (1991) Effect of diabetes mellitus induced by streptozotocin on renal superoxide dismutases in rat: Immunoreactivity and histochemical study.Virchow Arch. B 60, 67–72.
Hayashibe, H., Asayama, K., Dobashi, K. &Kato, K. (1990) Prenatal development of antioxidant enzymes in rat lung, kidney, and heart: Marked increase in immunoreactive superoxide dismutases, glutathione peroxidase, and catalase in the kidney.Pediatr. Res. 27, 472–5.
Jones, C. T. &Rolph, T. P. (1985) Metabolism during fetal life: A functional assessment of metabolic development.Physiol. Rev. 65, 357–430.
Munim, A., Asayama, K., Dobashi, K., Suzuki, K., Kawaoi, A. &Kato, K., (1992) Immunohistochemical localization of superoxide dismutases in fetal and neonatal rat tissues.J. Histochem. Cytochem. 40, 1705–13.
Murakoshi, M., Tagawa, M., Inada, R., Shoji, M., Suzuki, M. &Watanabe, K. (1992) The effects of new steroidal anti-androgen, TZP-4238, and chlormadinone acetate on the pituitary, prostate and adrenal gland of the rat: histopathological and immunocytochemical studies.Endocrinol. Jpn. 39, 331–40.
Muse, K. E., Oberley, T. D., Sempe, J. M. &Oberley, L. W. (1994) Immunolocalization of antioxidant enzymes in adult hamster kidney.Histochem. J. 26, 734–53.
Oberley, T. D., Oberley, L. W., Slattery, A. F. &Elwell, J. H. (1991) Immunohistochemical localization of gluatathione-Stransferase and glutathione peroxidase in adult Syrian hamster tissues and during kidney development.Am. J. Pathol.,139, 355–69.
Roveri, A., Casasco, A., Maiorino, M., Dalan, P., Calligaro, A. &Ursini, F. (1992) Phospholipid hydroperoxide glutathione peroxidase of rat testis.J. Biol. Chem. 267, 6142–6.
Sissman, N. J. (1970) Developmental landmarks in cardiac morphogenesis: Comparative chronology.Am. J. Cardiol. 25, 141–8.
Slot, J. W., Geuze, H. J., Freeman, B. A. &Crapo, J. D. (1986) Intracellular localization of the copper-zinc and manganese superoxide dismutases in the liver parenchymal cells.Lab. Invest. 55, 363–71.
Tauchi, K., Tsutsumi, Y., Tsukamoto, H., Hasegawa, H., Yoshimura, S. &Watanabe, K. (1991) Glutathione peroxidase and glutathione S-transferase, class α, in rat intestine.Acta Pathol. Jpn. 41, 573–80.
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Asayama, K., Dobashi, K., Kawada, Y. et al. Immunohistochemical localization and quantitative analysis of cellular glutathione peroxidase in foetal and neonatal rat tissues: Fluorescence microscopy image analysis. Histochem J 28, 63–71 (1996). https://doi.org/10.1007/BF02331428
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DOI: https://doi.org/10.1007/BF02331428