Skip to main content

Separation of fat-soluble vitamins by thin-layer chromatography

Chromatographia volume 9, pages 130–132 (1976)Cite this article

Summary

This paper is dealing with the evaluation of the method for qualitative analyses of fat soluble vitamins by thin-layer chromatography on impregnated starch, cellulose and talc thin layers. Impregnation was carried out with paraffin oil. Complete analyses could be obtained on two plates with two different solvents. Vitamins K3, K4 and K5 were separated with solvent I (water-dioxane-acetone-formaldehyde (85∶20∶15∶25)) whereas the other remain on the start position. Vitamins A-acetate, A-palmitate, K1, DL-alpha-tocopherol, DL-alpha-tocopherol-acetate, D2, D3 were separated with solvent II (acetone-concentrated acetic acid (30∶20)) while K3, K4 and K5 migrate with front. Quick and simple procedures for the preparation of the plates with an impregnated thin layer is given.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

39,95 €

Price includes VAT (Finland)

References

  1. J. Davidek andJ. Blattna, J. Chromatogr.7, 204 (1962).

    CAS  Google Scholar 

  2. H. K. Lichtenthaler, J. Chromatogr.13, 166 (1964).

    Article  CAS  Google Scholar 

  3. S. Ishikawa andG. Katsui, Vitamins (Kyoto)29, 203 (1964).

    CAS  Google Scholar 

  4. L. Cima, L. Levorato andR. Mantovan, Farmaco (Pavia), Ed. Prat.19, 428 (1964).

    CAS  Google Scholar 

  5. G. Katsui, Vitamins (Kyoto)29, 211 (1964).

    CAS  Google Scholar 

  6. K. V. John, M. R. Lankshmanan, F. B. Junalwala andH. R. Cama, J. Chromatogr.18, 53 (1965).

    CAS  Google Scholar 

  7. M. H. Hashmi, F. R. Chughtai andM. J. D. Chughtai, Mikrochim. Acta, 53 (1969).

  8. A. A. Artamonaova, Biokhim. Fiziol. Pitan. Zhivotn., 101, (1972); Chem. Abstr.78, 40000 s. (1973).

  9. H. Wardynska, K. Paczek andH. Galenska, Acta Pol. Pharm.30, 587 (1973).

    CAS  Google Scholar 

  10. D. Sklan andP. Budowski, Anal. Chem.45, 201 (1973).

    Google Scholar 

  11. G. W. Johnson andC. Vickers, Analyst (London)98, 257 (1973).

    CAS  Google Scholar 

  12. H. G. Lavelady, J. Chromatogr.78, 449 (1973).

    Google Scholar 

  13. V. D. Canić andN. U. Perišić-Janjić, Tehnika (Beograd)25, 330 (1970).

    Google Scholar 

  14. V. D. Canić andVojinović, Bull. Soc. Chim., Beograd36, 125 (1971).

    Google Scholar 

  15. S. M. Petrobic andS. E. Petrovic, J. Chromatogr.21, 313 (1966).

    Google Scholar 

  16. V. D. Canić, N. U. Perisić andM. J. Babin, Z. Anal. Chem.264, 415 (1973).

    Google Scholar 

  17. V. D. Canić andN. U. Perisić-Janjić, Z. Anal. Chem.265, 332 (1973).

    Google Scholar 

  18. S. M. Petrović andV. D. Canić, Mikrochim. Acta. 599 (1969).

  19. V. D. Canić andN. U. Perisić-Janjić, Z. Anal. Chem.270, 16 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Technology, University of Novi Sad, 21000, Novi Sad, Yugoslavia

    N. Perišić-Janjić, S. Petrović & P. Hadzić

Authors
  1. N. Perišić-Janjić
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Petrović
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Hadzić
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Perišić-Janjić, N., Petrović, S. & Hadzić, P. Separation of fat-soluble vitamins by thin-layer chromatography. Chromatographia 9, 130–132 (1976). https://doi.org/10.1007/BF02330381

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02330381

Keywords

  • Acetic
  • Cellulose
  • Chromatography
  • Analytical Chemistry
  • Starch