Summary
Retention of an extended set of flavonoid compounds by octyl, octadecyl, phenyl and cyanopropyl-bonded, reversed-phase columns, with methanol, as the organic mobile phase modifier and acetic acid as the acid modifier is reported. Solvent strengths and useful ranges for both isocratic and gradient elution are determined. Relative retention is found to be independent of methanol volume fraction in the mobile phase for all the columns examined. Correlations between retention on different columns, specific selectivity effects and their dependence on molecular structure are analyzed. Practical applications for separation and identification are discussed.
Similar content being viewed by others
References
J. B. Harborne, T. J. Mabry, H. Mabry (Eds.), The Flavonoids, Chapman and Hall, London, 1975.
J. B. Harborne, in:V. Cody, E. Middleton, Jr. andJ. B. Harborne (Eds.), “Plant Flavonoids in Biology and Medicine”, A.R. Liss, New York, 1986, pp. 15–24.
K. R. Markham, Techniques of Flavonoid Identification, Academic Press, London, 1982.
J. B. Harborne, in:E. Heftman (Ed.), “Chromatography — Part B: Applications” (J. Chromatogr. Library Series, Vol. 22B), Elsevier, Amsterdam, 1983, p. B407.
B. Hostettmann, M. Hostettmann, in:J. B. Harborne, T. J. Mabry (Eds.), “The Flavonoids, Advances in Research”, Chapman and Hall, London 1982.
F. Dondi, Y. D. Kahie, G. Lodi, M. Remelli, P. Reschiglian, C. Bighi, Anal. Chim. Acta191, 261 (1986).
F. Dondi, Y. D. Kahie, G. Lodi, P. Reschiglian, C. Pietrogrande, C. Bighi, G. P. Cartoni, Chromatographia23, 844 (1987).
F. Dondi, Y. D. Kahie, G. Lodi, G. Blo, C. Pietrogrande, P. Reschiglian, J. Chromatogr.461, 281 (1989).
F. Dondi, G. Blo, Y. D. Kahie, G. Lodi, C. Pietrogrande, P. Reschiglian, Chromatographia25, 423 (1988).
L. R. Snyder, J. W. Dolan, J. R. Gant, J. Chromatogr.165, 3 (1979).
J. W. Dolan, J. R. Grant, L. R. Snyder, J. Chromatogr.165, 31 (1979).
P. E. Antle, A. P. Goldberg, L. R. Snyder, J. Chromatogr.321, 1 (1985).
W. Melander, J. Stoveken, Cs. Horvath, J. Chromatogr.199, 35 (1980).
W. R. Melander, J. X. Huang, Cs. Horvath, R. W. Stout, J. J. DeStafano, Chromatographia20, 641 (1985).
L. W. Wulf, C. W. Nagel, J. Chromatogr.116, 271 (1976).
N. Tanaka, Y. Tokuda, K. Iwaguchi, M. Araki, J. Chromatogr.239, 761 (1982).
T. Hanai, J. Hubert, J. Chromatogr.291, 81 (1984).
J. L. Thus, J. C. Kraak, J. Chromatogr.320, 271 (1985)
J. S. Cantrell, in:V. Cody, E. Middleton, Jr. andJ. B. Harborne, (Eds.), “Plant Flavonoids in Biology and Medicine”, A.R. Liss, New York, 1986, pp. 391–394.
M. C. Etter, Z. Urbanczyk-Lipkowsko, S. Baer, P. F. Barbara, J. Mol Struct.144, 155 (1986).
J. Guang-Zhu, Y. Yuriko, T. Ken-ichi, Acta Cryst.C 46, 310 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kahie, Y.D., Pietrogrande, C., Mendez, M.I.M. et al. Effects of stationary phase structure on retention and selectivity in reversed-phase liquid chromatography of flavonoid compounds with methanol as organic modifier. Chromatographia 30, 447–452 (1990). https://doi.org/10.1007/BF02328515
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02328515