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The use of modified acrylic copolymers for the preparation of immunoadsorbents

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Summary

Copolymer beads from acrylonitrile and ethyl either butyl acrylate or vinyl acetate, with amino, hydroxyl or carboxyl groups were activated with glutaraldehyde or water-soluble carbodiimide and coupled to human serum albumin. The obtained immunoadsorbents were applied to the isolation of anti-human albumin anibody from rabbit antiserum. Among the 22 studied copolymers only a few showed satisfactory effectiveness for immunoaffinity chromatography, similar to that noted for immunoadsorbents from commerical polymers.

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Authors and Affiliations

  1. Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw

    A. Rapak & A. Szewczuk

  2. Institute of Organic and Polymer Technology, Technical University, 50-370, Wroclaw, Poland

    B. Kolarz & A. Trochimczuk

Authors
  1. A. Rapak
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  2. A. Szewczuk
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  3. B. Kolarz
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  4. A. Trochimczuk
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Rapak, A., Szewczuk, A., Kolarz, B. et al. The use of modified acrylic copolymers for the preparation of immunoadsorbents. Chromatographia 30, 428–431 (1990). https://doi.org/10.1007/BF02328511

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  • DOI: https://doi.org/10.1007/BF02328511

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