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Preparation of anhydrotrypsin-immobilized diol silica as a selective adsorbent for high-performance affinity chromatography of peptides containing arginine or lysine at their C-termini

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Summary

Anhydrotrypsin (AHT), a catalytically inert derivative of trypsin in which the active site serine residue was converted to dehydroalanine residue by chemical modification, was immobilized onto diol silica through the activation with trifluoroethanesulfonyl chloride, and an AHT-diol-silica column was used for high-performance affinity chromatography separation of peptides containing arginine or lysine at their C-termini from the others. Improved separation in terms of speed was accomplished.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara-Machi, Shinjuku-Ku, 162, Tokyo, Japan

    T. Ohta, T. Inoue, Y. Fukumoto & S. Takitani

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  1. T. Ohta
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  2. T. Inoue
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  3. Y. Fukumoto
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  4. S. Takitani
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Ohta, T., Inoue, T., Fukumoto, Y. et al. Preparation of anhydrotrypsin-immobilized diol silica as a selective adsorbent for high-performance affinity chromatography of peptides containing arginine or lysine at their C-termini. Chromatographia 30, 410–413 (1990). https://doi.org/10.1007/BF02328507

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  • DOI: https://doi.org/10.1007/BF02328507

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