Summary
Thin-layer chromatographic behaviour of thirteen anions on plain silica gel and silica gel impregnated with copper sulphate solution has been investigated in aqueous-organic solvents containing acetone, some of which have achieved reliable and reproducible separations. The effect of copper sulphate concentration on the mobility of anions has been examined. The results obtained on plain silica gel have been compared with those obtained on copper sulphate impregnated layers. The impregnated layers dramatically change the selectivity and permit separations not possible on untreated silica. Aqueous sodium chloride-acetone (9∶1) and ammonium hydroxide-acetone (9∶1) were the most effective solvent systems for differential migration of anions. Better results in terms of clarity of detection and compactness of spots were found with HCOOH-acetone as compared to HCl-acetone.
The effect of anion loading on RF values has been investigated and identification limits on impregnated layers determined.
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References
U. A. Th. Brinkman, G. De Vries, R. Kuroda, J. Chromatogr.,85, 187 (1973).
R. Kuroda, M.P. Volynets, CRC Handbook of Chromatography: Inorganics (M. Qureshi, ed.) Vol I, CRC press, Boca Raton, Fla, 1987.
A. Mohammad, K.G. Varshney, Handbook of Thin-Layer Chromatography (B. Fried andJ. Sherma, eds), Marcel Dekker, Inc., USA (in press).
J. Benes, Collect. Czech. Chem. Commun.,44, 1034 (1979).
J. Benes, Collect. Czech. Chem. Commun.,44, 1406 (1979).
H.M. Chawla, N.N. Ralhan, N.K. Garg, S.S. Chibber, J. High Res. Chromatogr./Chromatogr. Commun.,3, 651 (1980).
K. Kawanbe, K. Maruyama, Yakugaku Zusshi 1981, 101, 192; Chem. Abstr., 96, 45420K, 1982.
J. Zuanon Netto, A.F.C. Graner, M. Ionashiro, Ecletic Quim,9, 1984, 45; Chem. Abstr.,104, 141201u, 1986.
G. Buchbauer, R.E. Markis, Sci. Pharm.,51, 41 (1983).
R. Gallego, J.L. Bernal, A. Martinez, Quim Anal.,31, 3, 1977; Chem. Abstr., 1978,88, 1302267.
R. Gallego, J.L. Bernal, A. Martinez, Quim Anal.,31, 69, 1977; Chem. Abstr., 181811a, 1978.
K. Ravindra Nath, P.B. Janardhan, Chem. Abstr.,97, 65573m, 1982.
J. Franc, E. Kosikova, J. Chromatogr.,187, 462 (1980).
K. Sen, U.Ch. Ghosh, J. Liqd. Chromatogr.,3, 71 (1980).
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Mohammad, A., Tiwari, S. Thin-layer chromatographic separation and identification of some anions on copper sulphate impregnated silica gel layers. Chromatographia 30, 405–409 (1990). https://doi.org/10.1007/BF02328506
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DOI: https://doi.org/10.1007/BF02328506