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Immunochemistry of antigens from aerobically and anaerobically grownStaphylococcus aureus

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Abstract

Immunochemical analyses of the major cell-wall antigens (teichoic acid, mucopeptide, and protein A) demonstrated quantitative but not qualitative differences between these antigens prepared from aerobically and anaerobically grownStaphylococcus aureus 7167. The reduced rate of oxygen uptake observed in anaerobically grownS. aureus 7167, indicating a repressed electron transport system and a reduction in the number of proteins (enzymes) associated with this system, was interpreted as evidence for the significant reduction in the number of protein antigens observed in immunochemical analyses of cell homogenate and intracellular antigen fractions prepared from anaerobically grown cells. Immunoelectrophoretic analyses indicated that a quantitatively greater amount of polysaccharide capsular material was obtained from anaerobically grownS. aureus 7167, and a relationship betweenin vivo pathogenicity and anaerobiosis was proposed.

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Author notes

  1. T. C. O'Brien

    Present address: National Institutes of Health, Division of Biologics Standards, Laboratory of Virology and Rickettsiology, Department of Health, Education and Welfare, 20014, Bethesda, Maryland, USA

Authors and Affiliations

  1. Department of Biology, Catholic University of America, 20017, Washington, D.C., USA

    T. C. O'Brien & E. R. Kennedy

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O'Brien, T.C., Kennedy, E.R. Immunochemistry of antigens from aerobically and anaerobically grownStaphylococcus aureus . Antonie van Leeuwenhoek 38, 207–216 (1972). https://doi.org/10.1007/BF02328093

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